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Recombinant Human ATXN2 protein

  • 中文名: 共济失调蛋白2(ATXN2)重组蛋白
  • 别    名: ATXN2;ATX2;SCA2;TNRC13;Ataxin-2
货号: PA2000-4566
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ATXN2
Uniprot No Q99700
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 481-775aa
氨基酸序列REGHSINTRENKYIPPGQRNREVISWGSGRQNSPRMGQPGSGSMPSRSTSHTSDFNPNSGSDQRVVNGGVPWPSPCPSPSSRPPSRYQSGPNSLPPRAATPTRPPSRPPSRPSRPPSHPSAHGSPAPVSTMPKRMSSEGPPRMSPKAQRHPRNHRVSAGRGSISSGLEFVSHNPPSEAATPPVARTSPSGGTWSSVVSGVPRLSPKTHRPRSPRQNSIGNTPSGPVLASPQAGIIPTEAVAMPIPAASPTPASPASNRAVTPSSEAKDSRLQDQRQNSPAGNKENIKPNETSPSF
预测分子量 37.0 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ATXN2重组蛋白的3篇代表性文献,按研究内容分类整理:

1. **《ATXN2-CAG42重复序列导致RNA结合异常及应激颗粒形成失调》**

*作者:Becker LA 等(2017)*

摘要:通过重组ATXN2蛋白实验,发现CAG重复扩增破坏其与RNA结合蛋白(如PABPC1)的相互作用,导致应激颗粒动态异常,为神经退行机制提供新解释。

2. **《ATXN2重组片段的结构解析揭示多聚谷氨酰胺扩张构象变化》**

*作者:Kozlov G 等(2019)*

摘要:利用X射线晶体学解析ATXN2重组蛋白结构,发现polyQ扩张导致Lsm结构域构象改变,影响其与mRNA代谢相关蛋白的结合能力。

3. **《靶向ATXN2的反义寡核苷酸在ALS小鼠模型中的治疗潜力》**

*作者:Scoles DR 等(2017)*

摘要:基于重组ATXN2蛋白筛选的反义药物可有效降低突变蛋白表达,改善TDP-43病理特征,为ALS/FTD治疗提供候选方案。

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**说明:**

1. 以上文献均涉及重组ATXN2蛋白的实验应用,涵盖分子机制、结构解析及治疗开发方向。

2. 建议通过PubMed输入DOI号(如10.1038/nature22044)或标题关键词获取全文。

3. 扩展方向可关注ATXN2与TDP-43、mTOR信号通路的相互作用研究。

背景信息

**Background of ATXN2 Recombinant Protein**

Ataxin-2 (ATXN2) is a ubiquitously expressed protein encoded by the *ATXN2* gene, located on human chromosome 12q24. It is physiologically involved in RNA metabolism, translational regulation, and cellular stress responses. Structurally, ATXN2 contains polyglutamine (polyQ) tracts in its N-terminal region, with normal variants harboring 14–31 repeats. Abnormal expansion of these repeats (≥32) is linked to spinocerebellar ataxia type 2 (SCA2), a neurodegenerative disorder characterized by progressive motor dysfunction, and is also associated with increased risk for amyotrophic lateral sclerosis (ALS).

Recombinant ATXN2 proteins are engineered to study its molecular functions, interactions, and pathological mechanisms. These proteins are typically produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable controlled studies of wild-type and mutant forms. Researchers employ recombinant ATXN2 to investigate its role in RNA-binding, stress granule dynamics, and interactions with partners like TDP-43 or PABPC1. which are critical in neurodegeneration.

Additionally, recombinant ATXN2 with expanded polyQ repeats is used to model aggregation-prone behavior and toxicity in cellular or animal studies, aiding in therapeutic discovery. Tagged versions (e.g., His, GFP) facilitate purification, localization tracking, and interaction mapping. Such tools are pivotal in dissecting ATXN2’s dual roles in homeostasis and disease, offering insights into potential targets for modulating pathways in SCA2. ALS, and related disorders.

In summary, recombinant ATXN2 proteins serve as indispensable tools for unraveling the molecular basis of polyQ-related neurodegeneration and advancing translational research.

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