| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN |
| Entrez GeneID | 1786 |
| clone | 2D10G8 |
| WB Predicted band size | 183.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DNMT1 (AA: 1317-1616) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DNMT1抗体的3-4篇参考文献的简要概括：
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1. **"DNA methyltransferase 1 is essential for and uniquely regulates hematopoietic stem and progenitor cells"**
**作者**: Challen, G.A. et al.
**摘要**: 该研究利用DNMT1抗体在小鼠造血干细胞中检测其蛋白表达，发现DNMT1对维持干细胞自我更新和分化至关重要，缺失会导致DNA甲基化紊乱及干细胞耗竭。
2. **"Dynamic regulation of DNA methyltransferases in human cells"**
**作者**: Spada, F. et al.
**摘要**: 通过Western blot和免疫荧光(使用DNMT1特异性抗体)，揭示了DNMT1在细胞周期中的动态表达模式，并发现其与复制叉结合以维持甲基化模式的机制。
3. **"DNMT1 interacts with the G9a histone methyltransferase in cancer cells"**
**作者**: Hervouet, E. et al.
**摘要**: 研究通过免疫共沉淀(使用DNMT1抗体)发现DNMT1与组蛋白甲基转移酶G9a在癌症细胞中的相互作用，共同调控表观遗传沉默和肿瘤发生相关基因。
4. **"Antibody-based profiling of epigenetic markers in human cancers"**
**作者**: Gao, L. et al.
**摘要**: 该文献系统评估了多种表观遗传抗体(包括DNMT1抗体)的可靠性，发现其在多种癌症组织中的异常表达与患者预后显著相关，为诊断标志物开发提供依据。
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以上文献均通过DNMT1抗体在蛋白质检测、定位或互作研究中发挥作用，涵盖干细胞、表观调控及癌症等领域。
The DNA methyltransferase 1 (DNMT1) antibody is a key tool in epigenetic research, targeting the enzyme responsible for maintaining DNA methylation patterns during replication. DNMT1 ensures the faithful propagation of methylation marks—a critical process for gene silencing, genomic imprinting, and X-chromosome inactivation. Dysregulation of DNMT1 is linked to diseases like cancer, where aberrant methylation contributes to oncogene activation or tumor suppressor silencing, as well as neurodevelopmental disorders and aging-related conditions.
DNMT1 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to study DNMT1 expression, localization, and interactions. These antibodies often target specific regions, such as the N-terminal regulatory domain or C-terminal catalytic domain, enabling functional studies. Commercial DNMT1 antibodies vary in host species (e.g., rabbit, mouse), clonality (monoclonal vs. polyclonal), and applications, requiring validation for specificity via knockout controls or siRNA knockdown.
Research using DNMT1 antibodies has revealed its role in development, stem cell differentiation, and disease pathogenesis, making it a focal point for therapeutic targeting. Inhibitors of DNMT1. like decitabine, are already used clinically, underscoring the enzyme's biomedical relevance. Reliable DNMT1 antibodies remain essential for advancing epigenetic studies and translational drug development.
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