Mouse Monoclonal YWHAB Antibody

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

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     Western blot analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.    

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     Immunofluorescence analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

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     Immunofluorescence analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

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     Immunofluorescence analysis of HepG2 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

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     Flow cytometric analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).    

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     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using YWHAB mouse mAb with DAB staining.    

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     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using YWHAB mouse mAb with DAB staining.    

The YWHAB antibody is a crucial tool in biomedical research, specifically targeting the YWHAB protein, a member of the 14-3-3 protein family. The 14-3-3 proteins are highly conserved regulatory molecules expressed ubiquitously in eukaryotic cells, with seven isoforms (β, γ, ε, σ, ζ, η, τ) in humans. YWHAB, the beta isoform, plays a pivotal role in modulating cellular processes by binding to phosphorylated serine/threonine residues on diverse client proteins. This interaction regulates critical functions such as signal transduction, cell cycle progression, apoptosis, and metabolic pathways. Dysregulation of YWHAB has been implicated in neurological disorders, cancer, and metabolic diseases, underscoring its therapeutic and diagnostic relevance.

YWHAB antibodies are widely utilized in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect protein expression, localization, and interaction partners. They are essential for studying YWHAB's role in disease mechanisms, including its involvement in PI3K/AKT and MAPK signaling pathways. Additionally, these antibodies aid in exploring post-translational modifications of YWHAB that influence its binding specificity. Commercial YWHAB antibodies are typically validated for specificity across human and model organism samples (e.g., mouse, rat), with attention to cross-reactivity among 14-3-3 isoforms. Recent studies also highlight YWHAB's potential as a biomarker, driving demand for high-affinity, well-characterized antibodies in both research and clinical applications.