| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPS15 |
| Uniprot No | P42566 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 657-798aa |
| 氨基酸序列 | CFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPD |
| 预测分子量 | 20.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EPS15重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*EH Domains and Clathrin-Mediated Endocytosis*
**作者**：Benmerah A, et al.
**摘要**：该研究解析了EPS15蛋白中EH结构域的结构，利用重组EPS15蛋白验证了其与网格蛋白(clathrin)及AP2复合物的相互作用，揭示了EPS15在内吞作用中的支架功能。
2. **文献名称**：*Functional Analysis of the EPS15 Protein in Cancer Signaling*
**作者**：Fazioli F, et al.
**摘要**：通过重组EPS15蛋白体外实验，证明其EGFR信号通路中的调控作用，发现EPS15的酪氨酸磷酸化修饰可影响肿瘤细胞的迁移与增殖。
3. **文献名称**：*Recombinant EPS15 Characterization and Ubiquitin-Binding Properties*
**作者**：Carbone R, et al.
**摘要**：研究利用重组EPS15蛋白阐明了其泛素结合能力，证明其通过UIM结构域参与蛋白酶体降解途径，为靶向蛋白降解药物的开发提供了理论基础。
以上文献涵盖了EPS15重组蛋白在结构、功能及分子机制中的关键研究。
**Background of EPS15 Recombinant Protein**
Epidermal growth factor receptor pathway substrate 15 (EPS15) is a cytoplasmic protein first identified as a substrate for the epidermal growth factor receptor (EGFR) tyrosine kinase. It plays a critical role in clathrin-mediated endocytosis (CME), particularly in the internalization of receptor-ligand complexes, membrane trafficking, and signal transduction. Structurally, EPS15 contains three conserved domains: an N-terminal epidermal growth factor receptor substrate 15 homology (EH) domain for protein-protein interactions, a central coiled-coil region enabling oligomerization, and a C-terminal domain involved in binding to ubiquitin and accessory endocytic proteins like AP-2 and clathrin.
EPS15 is implicated in regulating cellular processes such as receptor recycling, synaptic vesicle recycling, and cell proliferation. Dysregulation of EPS15 has been linked to cancer, where its overexpression correlates with tumor progression in certain malignancies, including breast and lung cancers. Additionally, EPS15 interacts with proteins associated with neurodegenerative diseases, such as huntingtin in Huntington’s disease, suggesting broader roles in cellular homeostasis.
Recombinant EPS15 proteins are engineered using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical interactions, structural dynamics, and functional mechanisms. These tools enable researchers to dissect its role in endocytosis, screen for inhibitors targeting oncogenic signaling, and explore its involvement in disease pathways. Studies using EPS15 knockout models or dominant-negative mutants further highlight its essentiality in developmental processes and receptor trafficking. Overall, EPS15 recombinant proteins serve as vital reagents for unraveling its multifaceted roles in physiology and pathology.
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