| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAND1 |
| Uniprot No | O96004 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-215aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMNLVGSYAHHHHHHHPHPAHPMLHEPF LFGPASRCHQERPYFQSWLLSPADAAPDFPAGGPPPAAAAAATAYGPDAR PGQSPGRLEALGGRLGRRKGSGPKKERRRTESINSAFAELRECIPNVPAD TKLSKIKTLRLATSYIAYLMDVLAKDAQSGDPEAFKAELKKADGGRESKR KRELQQHEGFPPALGPVEKRIKGRTGWPQQVWALELNQ |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HAND1重组蛋白的3篇参考文献及其摘要概要：
---
1. **文献名称**：*"Recombinant HAND1 Protein Regulates Cardiac Lineage Commitment in Embryonic Stem Cells"*
**作者**：Smith J, Doe A, Lee C.
**摘要**：研究利用大肠杆菌表达系统成功纯化HAND1重组蛋白，并证明其通过结合特定DNA基序(如E-box)调控胚胎干细胞向心肌细胞分化的能力，揭示了其在心脏发育中的关键作用。
2. **文献名称**：*"Crystal Structure of the HAND1 bHLH Domain Reveals Dimerization Dynamics"*
**作者**：Zhang Y, Wang X, Tanaka M.
**摘要**：通过X射线晶体学解析HAND1重组蛋白的bHLH结构域，阐明其与DNA结合的构象变化及二聚化机制，为设计靶向HAND1的小分子提供结构依据。
3. **文献名称**：*"HAND1 Recombinant Protein Modulates Trophoblast Differentiation and Placental Development"*
**作者**：Chen L, Gupta R, Martínez P.
**摘要**：发现HAND1重组蛋白通过调控滋养层细胞分化相关基因(如HLA-G)影响胎盘形成，提示其在妊娠早期发育异常中的潜在病理机制。
---
**注**：以上文献信息为示例性质，实际引用需根据具体数据库检索结果调整。建议通过PubMed或Google Scholar以关键词“HAND1 recombinant protein”筛选最新研究。
HAND1 (Heart and Neural Crest Derivatives Expressed 1) is a member of the basic helix-loop-helix (bHLH) transcription factor family, playing critical roles in embryonic development, particularly in cardiogenesis and placental formation. It regulates cell differentiation, tissue patterning, and organogenesis by binding to DNA and modulating gene expression. During embryogenesis, HAND1 is essential for the development of the heart’s left ventricle, as well as trophoblast differentiation in the placenta. Dysregulation of HAND1 has been linked to congenital heart defects, miscarriages, and diseases like preeclampsia.
Recombinant HAND1 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. The protein is purified via affinity tags (e.g., His-tag) and chromatography techniques. Researchers use recombinant HAND1 to study its molecular interactions, DNA-binding properties, and regulatory mechanisms in vitro. It serves as a tool to investigate developmental pathways, model diseases caused by HAND1 mutations, or screen potential therapeutic compounds targeting HAND1-related disorders.
In cancer biology, HAND1’s role is context-dependent; it may act as a tumor suppressor or promoter. Recombinant HAND1 aids in exploring its dual functions in cancers like neuroblastoma or leukemia. Additionally, it is utilized in stem cell research to direct differentiation into cardiomyocytes or trophoblasts, supporting regenerative medicine approaches. Despite its significance, challenges remain in understanding HAND1’s dynamic interactions and tissue-specific effects. Ongoing studies aim to clarify its pleiotropic roles and therapeutic potential, bridging gaps between developmental biology and clinical applications.
×
