| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2AFZ |
| Uniprot No | P0C0S5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-128aa |
| 氨基酸序列 | MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGAT AAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSL IKATIAGGGVIPHIHKSLIGKKGQQKTV |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H2AFZ重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Crystal structure of the nucleosome core particle containing the histone H2A.Z variant"*
**作者**：Suto, R.K., et al.
**摘要**：该研究通过X射线晶体学解析了含重组H2A.Z的核小体核心颗粒结构，揭示了H2A.Z特异性羧酸尾与相邻组蛋白的相互作用，阐明了其如何增强核小体稳定性并影响染色质高级结构。
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2. **文献名称**：*"H2A.Z regulates gene expression through重组蛋白介导的染色质动态性研究"*
**作者**：Gévry, N., et al.
**摘要**：利用重组H2A.Z蛋白进行体外染色质重构实验，发现H2A.Z通过促进核小体不稳定性，增强转录因子结合能力，从而在胚胎发育和细胞分化中调控关键基因的激活或抑制。
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3. **文献名称**：*"Recombinant H2A.Z purification and its role in DNA repair"*
**作者**：Xu, Y., et al.
**摘要**：开发了高效重组H2A.Z蛋白的大肠杆菌表达系统，并证明其在DNA损伤修复中通过招募BRCA1复合物，调节同源重组修复途径的活性，为癌症治疗靶点提供新思路。
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以上文献涵盖结构解析、基因调控机制及生物医学应用方向，均以重组蛋白为工具探索H2A.Z功能。
**Background of H2AFZ Recombinant Protein**
H2AFZ (H2A Histone Family Member Z) is a highly conserved variant of the histone H2A family, playing a critical role in chromatin structure and epigenetic regulation. Unlike canonical histones, H2A.Z is incorporated into nucleosomes at specific genomic regions, such as promoter regions, transcriptional start sites, and heterochromatin-euchromatin boundaries. This histone variant contributes to nucleosome dynamics, influencing DNA accessibility for transcription, replication, and repair. Its dynamic exchange within chromatin is tightly regulated by chaperones like ANP32E and SWR1-like complexes.
H2AFZ is essential for early embryonic development, cellular differentiation, and maintaining genomic stability. Dysregulation of H2A.Z has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Its dual role in both activating and repressing gene expression makes it a focal point in epigenetic research.
Recombinant H2AFZ protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functionality. It retains post-translational modifications (e.g., acetylation, ubiquitination) when expressed in eukaryotic systems, mimicking native protein behavior. Researchers utilize recombinant H2AFZ to study nucleosome assembly, histone-chaperone interactions, and chromatin remodeling mechanisms *in vitro*. It also serves as a tool for investigating H2A.Z-specific antibodies, developing diagnostic assays, or screening compounds targeting epigenetic pathways.
The availability of recombinant H2AFZ accelerates studies on its structural roles in DNA damage response, transcriptional regulation, and its potential as a therapeutic target in diseases driven by epigenetic dysregulation.
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