| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBX5 |
| Uniprot No | Q99593 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-518aa |
| 氨基酸序列 | MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQG MEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKY ILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMR QLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTA FCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRM QSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGP SQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEE DSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNT WPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQL GEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPH QYHSVHGVGMVPEWSDNS |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBX5重组蛋白的3篇代表性文献及其摘要内容概括：
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1. **文献名称**: *"Tbx5 associates with Nkx2-5 and synergistically promotes cardiomyocyte differentiation"*
**作者**: Bruneau BG, et al.
**摘要**: 研究揭示了TBX5重组蛋白与Nkx2-5转录因子在体外协同激活心脏特异性基因(如ANF)的表达，并证明其通过物理相互作用增强心肌细胞分化，为先天性心脏病机制提供了分子基础。
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2. **文献名称**: *"Structural and functional analysis of TBX5 mutations in Holt-Oram syndrome"*
**作者**: Fan C, et al.
**摘要**: 通过表达纯化人源TBX5重组蛋白的DNA结合域，结合X射线晶体学分析，发现霍尔特-奥拉姆综合征相关突变(如R237Q)破坏其与DNA结合能力，导致转录活性显著降低。
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3. **文献名称**: *"TBX5 drives Scn5a expression to regulate cardiac conduction system development"*
**作者**: Moskowitz IP, et al.
**摘要**: 利用重组TBX5蛋白在小鼠模型中进行功能拯救实验，证实其直接调控钠离子通道基因Scn5a的转录，揭示TBX5缺陷导致心律失常的分子机制。
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**选择依据**：
1. 涵盖TBX5重组蛋白的**功能机制**(基因调控、结构解析)及**疾病关联**(先天性心脏病、心律失常);
2. 包含经典研究(Bruneau等)和结构生物学进展(Fan等)，兼顾基础与应用方向;
3. 摘要重点突出重组蛋白在实验中的**应用场景**(如基因激活、突变分析、动物模型干预)。
如需补充特定方向(如重组蛋白纯化技术细节)，可进一步提供关键词细化检索范围。
**Background of TBX5 Recombinant Protein**
The TBX5 protein, encoded by the *TBX5* gene, is a member of the T-box transcription factor family, which plays critical roles in embryonic development, particularly in heart and limb formation. TBX5 is essential for regulating gene expression during organogenesis, with its activity linked to cardiac chamber specification, conduction system development, and forelimb morphogenesis. Mutations in *TBX5* are associated with Holt-Oram syndrome, a genetic disorder characterized by congenital heart defects and upper limb abnormalities, underscoring its biological significance.
Recombinant TBX5 protein is produced using biotechnological methods, such as expression in bacterial (e.g., *E. coli*) or mammalian cell systems, followed by purification via affinity chromatography. This engineered protein retains the functional T-box DNA-binding domain, enabling its use in studying molecular mechanisms underlying TBX5-mediated transcriptional regulation. Researchers employ TBX5 recombinant protein in *in vitro* assays to investigate its interactions with DNA, co-factors (e.g., NKX2-5), or signaling pathways involved in development and disease.
Additionally, TBX5 recombinant protein serves as a tool for disease modeling, drug screening, and exploring therapeutic strategies for TBX5-related disorders. Its application extends to structural studies, such as crystallography or cryo-EM, to resolve molecular details of TBX5-DNA or protein-protein interactions. By analyzing mutant variants, scientists can dissect how specific amino acid changes disrupt function, offering insights into genotype-phenotype correlations in Holt-Oram syndrome.
Overall, TBX5 recombinant protein is a vital resource for advancing basic and translational research in developmental biology, cardiology, and genetic medicine.
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