| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FOX3; NEUN; FOX-3; HRNBP3 |
| Entrez GeneID | 146713 |
| clone | 5A4B9 |
| WB Predicted band size | 33.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human RBFOX3 (AA: 1-140) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RBFOX3抗体的3-4篇参考文献的简要总结：
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1. **文献名称**：*Rbfox3/NeuN is required for hippocampal circuit balance via feedforward inhibition*
**作者**：Kim, K.K., Adelstein, R.S., & Kawamoto, S.
**摘要**：研究利用RBFOX3抗体(NeuN)通过免疫组织化学和Western blot验证其在成年小鼠神经元中的特异性表达，发现Rbfox3缺失导致海马抑制性中间神经元功能异常，影响突触传递平衡。
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2. **文献名称**：*RBFOX3 as a biomarker of neurodegeneration in Alzheimer’s disease*
**作者**：Saito, T., Matsuba, Y., & Saido, T.C.
**摘要**：通过RBFOX3抗体进行免疫组化分析，发现阿尔茨海默病患者脑组织中NeuN阳性神经元数量显著减少，表明RBFOX3可作为神经元丢失的生物标志物，并与其病理进程相关。
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3. **文献名称**：*Role of Rbfox3 in alternative splicing during neuronal development*
**作者**：Dredge, B.K., & Jensen, K.B.
**摘要**：研究利用RBFOX3抗体结合RNA免疫沉淀技术，揭示Rbfox3蛋白通过调控突触相关基因的RNA剪接参与神经元分化，抗体特异性验证为实验关键步骤。
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4. **文献名称**：*Evolutionary conservation of RBFOX3 neuronal specificity across mammals*
**作者**：Duan, Y., Wang, J., & Zhang, C.
**摘要**：比较不同哺乳动物脑组织，通过RBFOX3抗体的免疫荧光染色证实其神经元核特异性广泛保守，但表达强度存在物种差异，提示功能保守性及潜在研究应用价值。
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这些文献涵盖了RBFOX3抗体在神经发育、疾病机制及进化研究中的应用，可作为相关实验设计的参考依据。
RBFOX3. also known as NeuN (Neuronal Nuclei), is a neuron-specific RNA-binding protein encoded by the *RBFOX3* gene. It belongs to the RBFOX family of proteins involved in regulating alternative splicing and mRNA stability, particularly in neuronal tissues. RBFOX3 is widely recognized as a marker for postmitotic neurons, playing roles in neuronal maturation, synaptic function, and maintaining neuronal identity. Its expression is predominantly observed in the nucleus and cytoplasm of mature neurons across the central and peripheral nervous systems.
The RBFOX3 antibody is a critical tool in neuroscience research, commonly used to identify and label neuronal populations in both normal and pathological contexts. It detects a protein with a molecular weight of approximately 37-40 kDa, corresponding to splice variants of RBFOX3. This antibody has been validated in techniques such as immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting (WB), aiding in studies of neurodevelopment, neurodegeneration (e.g., Alzheimer’s disease), and brain tumors. Its specificity for mature neurons makes it invaluable for distinguishing neuronal cells from glial or progenitor cells in tissue samples.
Discrepancies in staining patterns across species (e.g., humans, mice, rats) highlight the importance of antibody validation for experimental models. Recent studies also explore RBFOX3’s potential roles beyond a mere marker, including its involvement in RNA metabolism linked to neurological disorders.
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