| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FOX3; NEUN; FOX-3; HRNBP3 |
| Entrez GeneID | 146713 |
| clone | 1H3B2 |
| WB Predicted band size | 33.8kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human RBFOX3 (AA: 1-140) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RBFOX3抗体的3篇参考文献及其摘要：
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1. **文献名称**：*The neuronal nuclear protein NeuN is expressed in developing and adult neurons of the zebrafish*
**作者**：Kim KK, Adelstein RS, Kawamoto S
**摘要**：该研究验证了RBFOX3(NeuN)抗体在斑马鱼神经元中的特异性，证实其可用于标记发育和成熟神经元，并揭示NeuN在不同物种中的保守表达模式。
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2. **文献名称**：*Immunohistochemical markers for neuronal subpopulations in the human brain*
**作者**：Dredge BK, Jensen KB
**摘要**：本文评估了包括RBFOX3抗体在内的多种神经元标志物，发现其特异性受组织固定方法和抗原修复技术影响，强调了实验条件优化的重要性。
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3. **文献名称**：*NeuN as a marker of neuronal maturity in postmortem human brain*
**作者**：García-Cabezas MA, John YJ, Barbas H
**摘要**：研究利用RBFOX3抗体分析人脑尸检样本，发现NeuN表达水平与神经元成熟度相关，但在某些神经退行性疾病中表达下降，提示其作为病理标志物的潜力。
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**注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar核对最新研究。
RBFOX3. also known as neuronal nuclei (NeuN), is a RNA-binding protein encoded by the *RBFOX3* gene, primarily expressed in postmitotic neurons across the central and peripheral nervous systems. It belongs to the RBFOX family of proteins, characterized by a conserved RNA recognition motif (RRM) that regulates alternative splicing and transcriptional processing of target mRNAs. RBFOX3 plays a critical role in neuronal maturation, synaptic plasticity, and maintenance of neuronal function by modulating genes involved in axon guidance, ion channel activity, and cell signaling.
The RBFOX3 antibody is a widely used tool in neuroscience research to specifically identify mature neurons. It targets the NeuN antigen, a nuclear protein epitope expressed in most neuronal cell types, excluding cerebellar Purkinje cells, retinal photoreceptors, and subsets of hypothalamic neurons. First characterized in the 1990s, this antibody has become a gold-standard marker for neuronal identification in immunohistochemistry, immunofluorescence, and Western blotting. Its applications span neurodevelopmental studies, neurodegenerative disease research (e.g., Alzheimer's, Parkinson's), and investigations into neurological disorders such as epilepsy or schizophrenia. Recent studies also explore RBFOX3's potential role in neuronal stress responses and its altered expression patterns in brain tumors, highlighting its dual utility as both a diagnostic marker and a subject of functional investigation.
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