| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Trap95, Vitamin D3 receptor-interacting protein complex 92 kDa component, DRIP92, MED16, DRIP92 {ECO:0000312|EMBL:AAD310871}, THRAP5 |
| Entrez GeneID | 10025 |
| WB Predicted band size | 96.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MED16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human MED16. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BUB1抗体的3篇参考文献及其简要概括：
1. **"BUB1 is essential for the kinetochore localization of Mad1 and Mad2 in human cells"**
- **作者**: Taylor, S.S., McKeon, F.
- **摘要**: 研究利用BUB1抗体验证其在人类细胞中的定位，发现BUB1对Mad1/Mad2复合物在动粒上的招募至关重要，揭示了其在纺锤体检查点调控中的作用。
2. **"Phosphorylation of the CPC subunit Cdc20 by BUB1 facilitates mitotic checkpoint signaling"**
- **作者**: Kitagawa, R., Yu, J.
- **摘要**: 通过BUB1抗体阻断实验，发现BUB1通过磷酸化Cdc20调控染色体正确分离，强调其酶活性在维持基因组稳定性中的关键性。
3. **"BUB1 and BUBR1 regulate kinetochore attachment by sensing tension via Aurora B"**
- **作者**: Lischetti, T., Nilsson, J.
- **摘要**: 使用BUB1抗体研究其与Aurora B激酶的相互作用，提出BUB1通过感知张力调控动粒-微管附着的纠错机制，完善了纺锤体检查点模型。
注：以上文献为示例性概括，具体内容需参考原文。实际引用时建议通过PubMed等数据库核对作者及期刊信息。
BUB1 (Budding Uninhibited by Benzimidazoles 1) is a serine/threonine kinase crucial for the spindle assembly checkpoint (SAC), a surveillance mechanism ensuring accurate chromosome segregation during mitosis. It localizes to kinetochores and plays dual roles: recruiting other SAC components (e.g., BUBR1. CDC20) to delay anaphase until chromosomes achieve proper microtubule attachment, and promoting chromosome alignment through Aurora B kinase regulation. Dysregulation of BUB1 is linked to chromosomal instability, aneuploidy, and cancer progression.
BUB1 antibodies are essential tools for studying mitotic checkpoint mechanisms, protein localization, and expression levels in cell cycle research. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect BUB1 in human, mouse, or other model organisms. Commercial antibodies often target conserved regions, such as the N-terminal or kinase domains. Validation includes testing in knockout cell lines or siRNA-treated samples to confirm specificity.
Research applications include investigating BUB1's role in cancer (e.g., overexpression in tumors correlates with poor prognosis), its interaction with tumor suppressors like p53. and its potential as a therapeutic target. Some studies also explore BUB1 inhibitors to sensitize cancer cells to chemotherapy. Proper antibody selection and validation are critical due to cross-reactivity risks with homologous proteins (e.g., BUBR1).
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