| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FER |
| Uniprot No | P16591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-822aa |
| 氨基酸序列 | MGFGSDLKNSHEAVLKLQDWELRLLETVKKFMALRIKSDKEYASTLQNLCNQVDKESTVQMNYVSNVSKSWLLMIQQTEQLSRIMKTHAEDLNSGPLHRLTMMIKDKQQVKKSYIGVHQQIEAEMIKVTKTELEKLKCSYRQLIKEMNSAKEKYKEALAKGKETEKAKERYDKATMKLHMLHNQYVLALKGAQLHQNQYYDITLPLLLDSLQKMQEEMIKALKGIFDEYSQITSLVTEEIVNVHKEIQMSVEQIDPSTEYNNFIDVHRTTAAKEQEIEFDTSLLEENENLQANEIMWNNLTAESLQVMLKTLAEELMQTQQMLLNKEEAVLELEKRIEESSETCEKKSDIVLLLSQKQALEELKQSVQQLRCTEAKFSAQKELLEQKVQENDGKEPPPVVNYEEDARSVTSMERKERLSKFESIRHSIAGIIRSPKSALGSSALSDMISISEKPLAEQDWYHGAIPRIEAQELLKKQGDFLVRESHGKPGEYVLSVYSDGQRRHFIIQYVDNMYRFEGTGFSNIPQLIDHHYTTKQVITKKSGVVLLNPIPKDKKWILSHEDVILGELLGKGNFGEVYKGTLKDKTSVAVKTCKEDLPQELKIKFLQEAKILKQYDHPNIVKLIGVCTQRQPVYIIMELVSGGDFLTFLRRKKDELKLKQLVKFSLDAAAGMLYLESKNCIHRDLAARNCLVGENNVLKISDFGMSRQEDGGVYSSSGLKQIPIKWTAPEALNYGRYSSESDVWSFGILLWETFSLGVCPYPGMTNQQAREQVERGYRMSAPQHCPEDISKIMMKCWDYKPENRPKFSELQKELTIIKRKLT |
| 预测分子量 | 94,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与FER重组蛋白相关的研究文献摘要，供参考：
1. **文献名称**：Structural basis of FER tyrosine kinase activation and substrate recognition(作者：Chen et al., 2021)
**摘要**：本研究解析了重组FER激酶结构域的晶体结构，揭示了其自抑制构象及底物结合的关键位点，为FER在信号传导中的调控机制提供了结构生物学依据。
2. **文献名称**：FER kinase promotes cancer cell proliferation via YAP/TAZ signaling(作者：Li et al., 2019)
**摘要**：通过重组FER蛋白的功能实验，证明其通过磷酸化YAP/TAZ转录因子激活下游促癌基因表达，揭示了FER在肿瘤生长中的分子机制。
3. **文献名称**：Recombinant FER expression in E. coli and its role in cell-cell adhesion(作者：Wang & Zhang, 2016)
**摘要**：报道了在大肠杆菌中高效表达可溶性FER重组蛋白的方法，并验证其通过调控钙黏蛋白影响上皮细胞粘附功能的生物学活性。
4. **文献名称**：Targeting FER kinase in antiviral innate immunity(作者：Kim et al., 2020)
**摘要**：利用重组FER蛋白筛选小分子抑制剂，发现FER通过调控干扰素通路抑制病毒复制，提示其作为抗病毒治疗靶点的潜力。
注：上述文献为虚拟示例，实际研究中建议通过PubMed或Web of Science检索具体论文。
FER (FPS/FES-related) recombinant protein is derived from the FER tyrosine kinase, a member of the Fes/FER kinase family involved in diverse cellular processes. Discovered in the 1980s, FER is encoded by the FER gene located on human chromosome 5. It contains a conserved N-terminal FERM (F for FERM) domain, a central SH2 (Src homology 2) domain, and a C-terminal kinase domain, enabling roles in signal transduction, cell adhesion, and cytoskeletal regulation. FER interacts with signaling molecules like cadherins, integrins, and growth factor receptors, modulating immune responses, cell migration, and oncogenesis.
Recombinant FER protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional protein for research. Its production allows studies on kinase activity, substrate specificity, and signaling pathways. Researchers utilize it to investigate FER's role in diseases, particularly cancer, where aberrant FER expression correlates with tumor progression and metastasis. Additionally, it aids in developing inhibitors targeting FER's kinase domain for therapeutic applications. Recombinant FER also serves as an antigen for antibody generation, facilitating diagnostic tools and mechanistic studies. Overall, FER recombinant protein is a critical resource for decoding its biological functions and exploring clinical potential.
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