| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS4Y1 |
| Uniprot No | P22090 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263aa |
| 氨基酸序列 | MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIVFLRNRLKYALTGDEVKKICMQRFIKIDGKVRVDVTYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITVEEAKYKLCKVRKITVGVKGIPHLVTHDARTIRYPDPVIKVNDTVQIDLGTGKIINFIKFDTGNLCMVIGGANLGRVGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGNGNKPWISLPRGKGIRLTVAEERDKRLATKQSSG |
| 预测分子量 | 36.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于RPS4Y1重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: "Expression and Functional Analysis of Recombinant RPS4Y1 in Male-Specific Cellular Processes"
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了RPS4Y1蛋白，并验证其参与男性细胞中核糖体组装的功能，发现其缺失可能导致Y染色体相关翻译异常。
2. **文献名称**: "Structural Characterization of RPS4Y1 and Its Interaction with Ribosomal RNA"
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了RPS4Y1重组蛋白的三维结构，揭示其与18S rRNA的特异性结合位点，为理解性别差异相关的翻译机制提供结构基础。
3. **文献名称**: "RPS4Y1 Recombinant Protein as a Biomarker in Male-Specific Cancers"
**作者**: Tanaka K, et al.
**摘要**: 利用重组RPS4Y1蛋白开发了前列腺癌血清检测方法，证实其在男性肿瘤组织中的异常高表达与疾病进展显著相关。
4. **文献名称**: "Knockdown of RPS4Y1 via CRISPR/Cas9 Disrupts Spermatogenesis in Vitro"
**作者**: Gupta R, et al.
**摘要**: 通过重组蛋白互补实验证明，RPS4Y1在精子发生过程中调控特定mRNA的翻译效率，其功能缺失导致减数分裂停滞。
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**提示**：实际文献需通过PubMed、Web of Science等平台检索，建议使用关键词：
`RPS4Y1 recombinant protein`、`RPS4Y1 expression`、`Y-linked ribosomal protein function`
可重点关注《Gene》《PLOS ONE》《Scientific Reports》等期刊近年发表的相关研究。
**Background of RPS4Y1 Recombinant Protein**
RPS4Y1 (Ribosomal Protein S4 Y-Linked 1) is a Y-chromosome-encoded gene that belongs to the ribosomal protein S4 family. It plays a critical role in ribosomal assembly and protein synthesis as a component of the 40S ribosomal subunit. Unlike its X-linked homolog, RPS4X, RPS4Y1 is expressed predominantly in males due to its location on the Y chromosome. This gene exhibits evolutionary conservation across mammals, reflecting its essential function in cellular processes.
The RPS4Y1 protein is implicated in sex-specific differences in ribosomal function and gene expression regulation. Studies suggest it may contribute to cellular proliferation, apoptosis, and immune responses, though its non-ribosomal roles remain under investigation. Notably, RPS4Y1 has been linked to Turner syndrome and certain cancers, where dysregulated expression correlates with disease progression or therapy resistance.
Recombinant RPS4Y1 protein is produced using expression systems like *E. coli* or mammalian cells, enabling functional studies. Its purification allows researchers to explore interactions with RNA, other ribosomal proteins, or signaling molecules. Additionally, RPS4Y1 has been studied in autoimmune contexts, such as systemic lupus erythematosus (SLE), where antibodies targeting this protein are detected, suggesting its potential as a biomarker.
Research on RPS4Y1 recombinant protein also aids in understanding sex-biased diseases and developing targeted therapies. Its unique chromosomal origin and functional duality (ribosomal and extra-ribosomal) make it a compelling subject for both basic and translational studies.
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