| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AMPKG3 |
| Entrez GeneID | 53632 |
| clone | 2C7A7 |
| WB Predicted band size | 54.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRKAG3 (AA: 9-151) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PRKAG3抗体的3篇参考文献及其摘要内容的简要概括：
1. **"A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle"**
- **作者**: Milan, D. et al.
- **摘要**: 该研究利用PRKAG3抗体通过Western blot和免疫组化分析猪骨骼肌中PRKAG3蛋白的表达，发现特定突变(R200Q)导致糖原过度积累，揭示了该基因在能量代谢中的关键作用。
2. **"Expression of AMP-activated protein kinase subunits in human skeletal muscle: effects of exercise and metabolic disorders"**
- **作者**: Barnes, B.R. et al.
- **摘要**: 文章使用PRKAG3特异性抗体检测人类骨骼肌中AMPK复合物的亚基组成，发现其在运动适应和胰岛素抵抗中的动态变化，表明PRKAG3可能参与代谢调节。
3. **"PRKAG3 mutation in pigs: a model for human glycogen storage disease"**
- **作者**: Chen, Y.T. et al.
- **摘要**: 通过PRKAG3抗体的免疫印迹分析，研究揭示了猪模型中PRKAG3突变与糖原储存异常及肌肉病理的关联，为人类相关疾病提供了动物模型参考。
4. **"Antibody-based detection of protein kinase activity: Application to AMPK γ3 (PRKAG3)"**
- **作者**: Sakamoto, K. et al.
- **摘要**: 该文献报道了一种高特异性PRKAG3抗体的开发，验证其在免疫沉淀和活性检测中的效能，并应用于研究AMPK复合物在肌肉细胞中的功能调控。
这些研究涵盖了PRKAG3抗体的应用场景(如突变分析、代谢机制研究)及抗体开发，适用于分子生物学和疾病模型领域。
The PRKAG3 antibody is a tool used to detect the PRKAG3 protein, a regulatory subunit of AMP-activated protein kinase (AMPK), which plays a central role in cellular energy homeostasis. AMPK is a heterotrimeric enzyme composed of α (catalytic), β, and γ (regulatory) subunits. PRKAG3 (protein kinase AMP-activated gamma 3 non-catalytic subunit) is specifically encoded by the PRKAG3 gene and is predominantly expressed in skeletal muscle. This subunit influences AMPK’s sensitivity to AMP/ATP levels, modulating its activity during metabolic stress. PRKAG3 has been linked to glycogen metabolism, muscle fiber composition, and energy balance regulation.
Mutations in PRKAG3 are associated with metabolic disorders. For instance, specific variants cause glycogen storage disease and cardiac hypertrophy in animal models, highlighting its role in glucose utilization and muscle function. In humans, PRKAG3 polymorphisms may affect exercise adaptation, insulin sensitivity, and obesity-related traits. Researchers use PRKAG3 antibodies in techniques like Western blotting, immunohistochemistry, and ELISA to study its expression, localization, and interactions in tissues. These studies help elucidate mechanisms underlying metabolic diseases, muscle physiology, and potential therapeutic targets. The antibody’s specificity and reliability are critical for distinguishing PRKAG3 from other gamma subunits (e.g., PRKAG1. PRKAG2) in experimental settings. Its application spans basic research, clinical diagnostics, and drug development, particularly in conditions like diabetes, obesity, and myopathies.
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