| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMP2K |
| Uniprot No | Q9NSY1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 39-344aa |
| 氨基酸序列 | VGVRVFAVGRHQVTLEESLAEGGFSTVFLVRTHGGIRCALKRMYVNNMPDLNVCKREITIMKELSGHKNIVGYLDCAVNSISDNVWEVLILMEYCRAGQVVNQMNKKLQTGFTEPEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLNDGGNYVLCDFGSATNKFLNPQKDGVNVVEEEIKKYTTLSYRAPEMINLYGGKPITTKADIWALGCLLYKLCFFTLPFGESQVAICDGNFTIPDNSRYSRNIHCLIRFMLEPDPEHRPDIFQVSYFAFKFAKKDCPVSNINNSSIPSALPEPMTAS |
| 预测分子量 | 41.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BMP2K重组蛋白的模拟参考文献示例(请注意，具体文献需通过学术数据库验证)：
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1. **标题**: *"Cloning and Functional Characterization of Recombinant BMP2K in Osteogenic Differentiation"*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化重组BMP2K蛋白，证实其具有激酶活性，并通过磷酸化SMAD1/5/9调控成骨细胞分化，为骨再生研究提供工具。
2. **标题**: *"Structural Insights into BMP2K Kinase Domain and Its Role in BMP Signaling"*
**作者**: Zhang L, et al.
**摘要**: 通过X射线晶体学解析BMP2K激酶结构域的三维结构，揭示其与BMP-II型受体的相互作用机制，阐明其在BMP信号转导中的关键作用。
3. **标题**: *"Recombinant BMP2K Production in HEK293 Cells: Optimization and Application in Drug Screening"*
**作者**: Tanaka K, et al.
**摘要**: 优化HEK293细胞中BMP2K重组蛋白的高效表达体系，开发基于其激酶活性的高通量药物筛选平台，用于靶向骨代谢疾病的抑制剂发现。
4. **标题**: *"BMP2K Knockdown and Recombinant Protein Rescue in Zebrafish Model"*
**作者**: Chen R, et al.
**摘要**: 在斑马鱼模型中，BMP2K缺失导致骨骼发育异常，外源添加重组BMP2K蛋白部分恢复表型，证明其在脊椎动物骨发育中的保守功能。
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**提示**：以上为模拟文献，实际研究中建议通过PubMed、Google Scholar等平台以“BMP2K recombinant protein”、“BMP2 kinase”等关键词检索最新文献。若BMP2K为特定研究组命名，需结合上下文确认其指代。
**Background of BMP2K Recombinant Protein**
BMP2K (BMP-2-Inducible Protein Kinase), also known as BIKe or STK35. is a serine/threonine kinase implicated in cellular signaling pathways regulated by bone morphogenetic proteins (BMPs), particularly BMP-2. BMPs are multifunctional growth factors belonging to the TGF-β superfamily, playing critical roles in embryonic development, osteogenesis, and tissue homeostasis. BMP2K is transcriptionally activated in response to BMP-2 stimulation and participates in downstream signaling cascades that modulate cell differentiation, proliferation, and apoptosis.
The recombinant BMP2K protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. It is purified via affinity chromatography, retaining functional activity for experimental applications. Researchers utilize BMP2K recombinant protein to study its role in BMP-mediated pathways, particularly in osteoblast differentiation, bone regeneration, and diseases linked to BMP signaling dysregulation, such as fibrosis, cancer, and skeletal disorders.
Recent studies highlight BMP2K’s interaction with cytoskeletal components and its potential influence on cell motility and adhesion, suggesting broader implications in wound healing and metastasis. Its recombinant form serves as a vital tool for *in vitro* kinase assays, structural studies, and therapeutic exploration, offering insights into targeting BMP-related pathologies. Despite progress, the full mechanistic scope of BMP2K remains under investigation, emphasizing its significance in both basic and translational research.
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