| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HES7 |
| Uniprot No | Q9BYE0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-225aa |
| 氨基酸序列 | MVTRDRAENR DGPKMLKPLV EKRRRDRINR SLEELRLLLL ERTRDQNLRN PKLEKAEILE FAVGYLRERS RVEPPGVPRS PVQDAEALAS CYLSGFRECL LRLAAFAHDA SPAARAQLFS ALHGYLRPKP PRPKPVDPRP PAPRPSLDPA APALGPALHQ RPPVHQGHPS PRCAWSPSLC SPRAGDSGAP APLTGLLPPP PPPHRQDGAP KAPLPPPPAF WRPWP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HES7重组蛋白的3篇虚构参考文献示例，格式包含文献名称、作者及摘要概括：
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1. **文献名称**：*Recombinant HES7 Protein Production in E. coli and Its Role in Notch Signaling Inhibition*
**作者**：Smith, J., et al.
**摘要**：本研究利用大肠杆菌表达系统成功克隆并纯化了HES7重组蛋白。通过体外实验证实，重组HES7能够特异性结合Notch信号通路的靶基因启动子区域，抑制下游基因转录，为研究体节发育机制提供了分子工具。
2. **文献名称**：*Structural and Functional Analysis of HES7 Recombinant Protein in Mammalian Cells*
**作者**：Lee, S., & Zhang, Y.
**摘要**：作者在HEK293细胞中表达了带有His标签的HES7重组蛋白，并通过X射线晶体学解析其三维结构。功能实验表明，该蛋白通过形成同源二聚体调控神经干细胞的分化，为癌症中Notch通路的异常激活提供了潜在干预靶点。
3. **文献名称**：*Development of a HES7-Based Biosensor for High-Throughput Drug Screening*
**作者**：Garcia, R., et al.
**摘要**：本研究开发了一种基于重组HES7蛋白的荧光报告系统，用于快速筛选调控Notch信号通路的小分子化合物。该方法在乳腺癌细胞模型中验证了HES7对肿瘤生长的抑制作用，为药物研发提供了新策略。
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注：以上文献为示例性虚构内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
**Background of HES7 Recombinant Protein**
HES7 (Hairy and Enhancer of Split 7) is a member of the basic helix-loop-helix (bHLH) family of transcriptional repressors, primarily known for its role in regulating developmental processes via the Notch signaling pathway. It functions as a key mediator of somitogenesis, the segmentation of the embryonic mesoderm into somites, which later contribute to skeletal muscle, vertebrae, and dermis. HES7 exhibits oscillatory expression patterns critical for maintaining the precision of the "segmentation clock," a molecular mechanism that controls the rhythmic formation of somites during vertebrate embryogenesis.
Recombinant HES7 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems, to produce a purified, biologically active form. This allows researchers to study its structure, DNA-binding properties, and interactions with co-repressors like Groucho/TLE proteins. Studies using recombinant HES7 have elucidated its role in repressing target genes, such as *Mesp2*, by binding to promoter regions and recruiting chromatin-modifying complexes.
Dysregulation of HES7 is linked to congenital disorders, including spondylocostal dysostosis, characterized by vertebral malformations. Recombinant HES7 serves as a tool for *in vitro* assays, drug screening, and disease modeling, offering insights into developmental biology and potential therapeutic strategies. Its conserved function across species, from zebrafish to humans, underscores its evolutionary significance in segmentation and tissue patterning.
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