| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DNM2;DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB |
| Entrez GeneID | 1785 |
| clone | 3F5C7 |
| WB Predicted band size | 98kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Dynamin-2 (AA: 520-744) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与Dynamin-2抗体相关的参考文献(信息为模拟生成，实际引用请核实原文)：
1. **标题**：Dynamin-2 mutations linked to centronuclear myopathy disrupt membrane remodeling in myotubes
**作者**：S. Dowling et al.
**摘要**：研究通过特异性Dynamin-2抗体发现，该蛋白突变导致肌管细胞膜结构异常，揭示了其在肌肉细胞膜重塑中的关键作用，与中央核肌病发病机制相关。
2. **标题**：Dynamin-2 regulates postsynaptic cytoskeleton organization via interaction with cortactin
**作者**：K. Nakatsu et al.
**摘要**：利用Dynamin-2抗体进行免疫共沉淀实验，证明其与皮层蛋白cortactin相互作用，调控突触后细胞骨架动态，影响神经元突触可塑性。
3. **标题**：Antibody-based targeting of Dynamin-2 inhibits clathrin-mediated endocytosis in cancer cells
**作者**：M. Chen & R. Weissleder
**摘要**：开发了一种功能性Dynamin-2单克隆抗体，证明其可特异性阻断网格蛋白介导的内吞作用，为肿瘤细胞内吞通路调控提供了实验工具与潜在治疗策略。
注：实际文献需通过PubMed/Google Scholar检索关键词"Dynamin-2 antibody" + "application"/"function"等获取，建议结合具体研究场景筛选。
Dynamin-2 (DNM2) is a large GTPase enzyme critical for membrane remodeling processes, particularly clathrin-mediated endocytosis and intracellular vesicle trafficking. It plays a key role in severing membrane-bound vesicles from the plasma membrane or organelles by forming helical polymers that constrict upon GTP hydrolysis. DNM2 is ubiquitously expressed and interacts with proteins like amphiphysin, endophilin, and microtubules. Dysregulation or mutations in the *DNM2* gene are linked to genetic disorders such as Charcot-Marie-Tooth disease (CMT), centronuclear myopathy, and hereditary spastic paraplegia.
Antibodies targeting Dynamin-2 are essential tools for studying its expression, localization, and function in cellular pathways. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation, helping researchers investigate DNM2’s role in physiological processes (e.g., synaptic transmission, receptor internalization) and disease mechanisms. Specific DNM2 antibodies may distinguish between isoforms or post-translationally modified forms, aiding in mechanistic studies. Commercially available antibodies are typically raised in rabbits or mice using immunogenic peptide sequences from conserved regions of the protein. Validation includes testing knockout cell lines or siRNA-mediated knockdown to confirm specificity. These antibodies are pivotal in exploring DNM2-associated pathologies and potential therapeutic targets, particularly in neuromuscular disorders.
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