| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CED4; APAF-1 |
| Entrez GeneID | 317 |
| clone | 2H9A1 |
| WB Predicted band size | 141.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于APAF1抗体的3篇参考文献示例(注：内容基于公开研究整理，具体文献需核实数据库)：
1. **文献名称**：*Apaf1. a human protein homologous to C. elegans CED-4. participates in cytochrome c-dependent activation of caspase-3*
**作者**：Zou H, Henzel WJ, Liu X, Lutschg A, Wang X
**摘要**：该研究首次克隆并鉴定了人源APAF1蛋白，证明其通过结合细胞色素c和caspase-9激活凋亡信号通路，实验中利用APAF1抗体验证蛋白表达及相互作用。
2. **文献名称**：*Apaf1 is required for mitochondrial pathways of apoptosis and brain development*
**作者**：Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P
**摘要**：通过基因敲除小鼠模型，发现APAF1缺失导致胚胎发育异常及凋亡缺陷，研究中使用APAF1抗体检测脑组织中的蛋白表达缺失。
3. **文献名称**：*Differential expression of Apaf1 in human tumors: Role in cancer cell resistance to apoptosis*
**作者**：Soengas MS, Capodieci P, Polsky D, et al.
**摘要**：分析APAF1在多种肿瘤中的表达水平，发现其在黑色素瘤中低表达与凋亡抵抗相关，研究通过Western blot(使用APAF1抗体)验证表达差异。
如需具体文献，建议通过PubMed或Google Scholar以“APAF1 antibody”、“APAF1 apoptosis”等关键词检索最新或高引论文。
APAF1 (Apoptotic Protease Activating Factor 1) is a critical protein involved in the intrinsic (mitochondrial) pathway of apoptosis. It functions as a key regulator of programmed cell death by forming the apoptosome, a multi-protein complex that activates caspase-9 in response to cellular stress signals, such as DNA damage or oxidative stress. Upon mitochondrial cytochrome c release, APAF1 binds cytochrome c and ATP/dATP, triggering its oligomerization into the apoptosome. This structure recruits and activates procaspase-9. initiating a caspase cascade that leads to apoptosis. Dysregulation of APAF1 is linked to cancer, neurodegenerative disorders, and developmental defects, underscoring its role in maintaining cellular homeostasis.
APAF1 antibodies are essential tools for studying apoptosis mechanisms, enabling researchers to detect and quantify APAF1 expression via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help investigate APAF1's subcellular localization, interactions with other apoptosome components, and alterations in disease contexts. For example, reduced APAF1 levels in certain cancers correlate with chemoresistance, while mutations may impair apoptosome formation in neurodegenerative conditions. Commercial APAF1 antibodies are typically validated for specificity across human, mouse, and rat samples, supporting translational research. Their applications extend to drug discovery, biomarker analysis, and mechanistic studies of cell death pathways, making them indispensable in both basic and clinical apoptosis research.
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