| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CSF3R; SCN7; GCSFR |
| Entrez GeneID | 1441 |
| clone | 8F8B12 |
| WB Predicted band size | 92.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD114 (AA: extra 25-187) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于CD114抗体的参考文献示例(内容为模拟生成，仅供参考)：
1. **"Development and characterization of a monoclonal antibody specific for human CD114 (GM-CSFRα)"**
- 作者：Smith A, et al.
- 摘要：该研究报道了一种新型抗CD114单克隆抗体的开发，验证了其在流式细胞术和免疫组化中对GM-CSFRα的特异性识别，并应用于白血病细胞表面受体表达分析。
2. **"CD114 expression correlates with poor prognosis in acute myeloid leukemia"**
- 作者：Johnson B, et al.
- 摘要：通过CD114抗体检测AML患者样本，发现CD114高表达与化疗耐药性和生存率降低相关，提示其作为预后标志物的潜力。
3. **"Targeting GM-CSFRα with anti-CD114 antibodies ameliorates inflammatory arthritis in murine models"**
- 作者：Lee C, et al.
- 摘要：研究利用抗CD114抗体阻断GM-CSF信号通路，显著减轻小鼠模型中关节炎症和骨侵蚀，为自身免疫性疾病治疗提供新策略。
4. **"A humanized anti-CD114 antibody enhances NK cell-mediated cytotoxicity against solid tumors"**
- 作者：Wang D, et al.
- 摘要：开发了人源化CD114抗体，通过增强NK细胞对肿瘤细胞的ADCC效应，在临床前实验中显示出抗实体瘤活性。
(注：以上文献为示例，实际引用请核对真实数据库。)
CD114. also known as the granulocyte-macrophage colony-stimulating factor receptor (GM-CSFR) β-chain or CSF2RB, is a cell surface protein that forms part of the receptor complex for GM-CSF, a cytokine critical for hematopoiesis and immune regulation. The GM-CSFR consists of a ligand-specific α-chain (CD116) and a signal-transducing β-chain (CD114), which is shared with receptors for interleukin-3 (IL-3) and interleukin-5 (IL-5). CD114 mediates intracellular signaling via JAK-STAT, PI3K, and MAPK pathways, influencing cell survival, proliferation, and differentiation of myeloid lineages, particularly granulocytes and macrophages.
CD114 antibodies are tools used to study GM-CSF signaling in contexts like hematologic disorders, inflammatory diseases, and cancer. Dysregulated GM-CSF/CD114 signaling is implicated in autoimmune conditions (e.g., rheumatoid arthritis), inflammatory syndromes (e.g., alveolar proteinosis), and myeloid malignancies. Therapeutic anti-CD114 antibodies, often designed to block GM-CSF binding or receptor dimerization, have been explored to dampen pathogenic inflammation or disrupt cancer cell survival. For example, mavrilimumab, a monoclonal antibody targeting the GM-CSFR α-chain, has shown efficacy in autoimmune trials, highlighting the potential of targeting this pathway. However, CD114-specific antibodies require careful evaluation due to risks of immunosuppression or hematologic toxicity. Research continues to clarify CD114's role in disease microenvironments and its utility as a diagnostic or therapeutic target.
×
