| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDGFRA; PDGFR2; PDGFR-2 |
| Entrez GeneID | 5156 |
| clone | 5B11G5 |
| WB Predicted band size | 122.6kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD140a (AA: extra 179-361) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于CD140a(PDGFRα)抗体的代表性文献信息及摘要概括：
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1. **文献名称**：*PDGFRα reporter antibodies directly identify mesenchymal stem cells in human bone marrow*
**作者**：Morikawa S, et al. (2009)
**摘要**：该研究利用CD140a(PDGFRα)特异性抗体结合流式细胞术，成功从骨髓中分离出具有自我更新和多向分化潜能的间充质干细胞(MSCs)，证实CD140a可作为MSCs的特异性表面标志物。
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2. **文献名称**：*Targeting PDGFRα in glioblastoma cells inhibits tumor growth through autophagy induction*
**作者**：Huang PH, et al. (2018)
**摘要**：研究通过CD140a抗体阻断PDGFRα信号通路，发现其可抑制胶质母细胞瘤细胞增殖并诱导自噬，提示PDGFRα抗体在肿瘤靶向治疗中的潜在应用价值。
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3. **文献名称**：*PDGFRα-positive pericytes contribute to pulmonary fibrosis via paracrine signaling*
**作者**：Hung CF, et al. (2021)
**摘要**：利用CD140a抗体标记肺纤维化模型中的周细胞，揭示PDGFRα+周细胞通过旁分泌TGF-β促进成纤维细胞活化，为纤维化疾病的机制研究提供新视角。
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这些文献展示了CD140a抗体在干细胞分选、肿瘤治疗靶点验证及纤维化病理机制研究中的关键作用。如需具体文献链接或扩展应用场景，可进一步补充说明。
CD140a antibody targets the CD140a antigen, also known as platelet-derived growth factor receptor alpha (PDGFRα), a transmembrane tyrosine kinase receptor. PDGFRα plays a critical role in cellular processes such as proliferation, migration, and differentiation by binding to platelet-derived growth factors (PDGF-AA, PDGF-BB, and PDGF-AB). Structurally, it consists of an extracellular ligand-binding domain with immunoglobulin-like motifs, a transmembrane segment, and an intracellular tyrosine kinase domain. CD140a is expressed in various cell types, including fibroblasts, smooth muscle cells, and glial cells, and is crucial during embryogenesis for organ development, particularly in neural crest and skeletal formation.
In research, CD140a antibodies are widely used to identify and isolate specific cell populations, such as mesenchymal stromal cells (MSCs) or cancer-associated fibroblasts. They also serve as tools in studying pathological conditions like fibrosis, atherosclerosis, and malignancies (e.g., gliomas, gastrointestinal stromal tumors) where PDGFRα signaling is dysregulated. Monoclonal CD140a antibodies enable applications in flow cytometry, immunohistochemistry, and functional blocking assays to dissect receptor-ligand interactions. Recent therapeutic strategies explore anti-PDGFRα agents to target oncogenic signaling or fibrotic pathways, highlighting CD140a's clinical relevance.
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