| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ACAS2L; ACECS1; AceCS2L |
| Entrez GeneID | 84532 |
| clone | 3A7C1 |
| WB Predicted band size | 74.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ACSS1 (AA: 548-689) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下为3-4篇与ACSS1抗体相关研究的代表性文献摘要：
1. **文献名称**：The mitochondrial acetyl-CoA synthetase ACSF2 is essential for cell growth in mammalian cells
**作者**：Luong A et al.
**摘要**：本研究通过CRISPR筛选和ACSS1/ACSF2特异性抗体验证，揭示了线粒体型乙酰辅酶A合成酶ACSF2对哺乳动物细胞增殖的关键作用，其缺失导致组蛋白乙酰化水平下降和细胞周期阻滞。
2. **文献名称**：ACSS1 regulates metabolic homeostasis under low-energy stress in glioblastoma
**作者**：Li X et al.
**摘要**：利用ACSS1特异性抗体进行免疫印迹和免疫荧光，发现胶质母细胞瘤在低营养胁迫下通过ACSS1介导的乙酸代谢维持细胞存活，敲低ACSS1显著抑制肿瘤生长。
3. **文献名称**：Acetylation of ACSS1 regulates its subcellular localization and metabolic function
**作者**：Sasaki T et al.
**摘要**：通过免疫共沉淀结合ACSS1抗体，发现其赖氨酸位点乙酰化修饰影响线粒体定位，进而调控肝癌细胞中脂肪酸合成的代谢重编程过程。
4. **文献名称**：ACSS1 as a prognostic biomarker in hepatocellular carcinoma
**作者**：Wang Y et al.
**摘要**：采用ACSS1多克隆抗体对肝癌组织进行免疫组化分析，发现ACSS1高表达与患者生存期缩短显著相关，其过表达促进肿瘤细胞脂质蓄积和侵袭转移能力。
注：上述内容为模拟文献概括，实际研究中建议通过PubMed(PMID检索)或Web of Science平台获取具体文献数据，使用关键词"ACSS1 antibody"+"cell metabolism"+"cancer"等组合筛选。
**Background of ACSS1 Antibody**
ACSS1 (acetyl-CoA synthetase 1) is a mitochondrial enzyme that catalyzes the conversion of acetate into acetyl-CoA, a critical substrate for energy production, lipid synthesis, and cellular metabolism. It plays a vital role in maintaining acetyl-CoA pools under low-glucose conditions, particularly in tissues reliant on mitochondrial respiration, such as the heart, liver, and brain. Dysregulation of ACSS1 has been implicated in metabolic disorders, cancer, and neurodegenerative diseases, highlighting its importance in cellular energy homeostasis.
The ACSS1 antibody is a specialized tool used to detect and quantify ACSS1 protein levels in research applications, including Western blotting, immunohistochemistry, and immunofluorescence. It aids in studying ACSS1’s expression patterns, subcellular localization, and functional roles in metabolic pathways. Validated antibodies ensure specificity through knockout controls, enabling researchers to explore ACSS1’s involvement in diseases like hepatocellular carcinoma, where its downregulation correlates with poor prognosis, or in neurological conditions linked to metabolic dysfunction.
As interest in metabolic reprogramming grows, particularly in cancer and aging, the ACSS1 antibody serves as a key reagent for elucidating mechanisms connecting acetate metabolism to cellular health and disease progression.
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