| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HS; NIK; HSNIK; FTDCR1B |
| Entrez GeneID | 9020 |
| clone | 2G2B4 |
| WB Predicted band size | 104kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAP3K14 (AA: 769-947) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于MAP3K14抗体的3篇参考文献示例(虚构内容，仅供格式参考)：
1. **"MAP3K14 regulates NF-κB signaling in B-cell malignancies"**
*Authors: Smith J, et al. (2019)*
**摘要**: 本研究利用MAP3K14抗体通过Western blot和免疫组化分析，揭示了MAP3K14在B细胞淋巴瘤中异常激活，促进NF-κB通路依赖的肿瘤生存。
2. **"NIK (MAP3K14) antibody validation in non-canonical NF-κB pathway studies"**
*Authors: Lee H, et al. (2017)*
**摘要**: 文章系统验证了多种MAP3K14抗体的特异性，通过敲除细胞模型和免疫沉淀实验，证实其在检测内源性NIK蛋白及磷酸化状态中的可靠性。
3. **"Targeting MAP3K14 in inflammatory bowel disease"**
*Authors: Chen R, et al. (2021)*
**摘要**: 研究使用MAP3K14抗体检测结肠组织中的蛋白表达，发现NIK水平与疾病严重程度相关，抑制MAP3K14可缓解小鼠模型炎症反应。
*注：以上文献为示例，实际引用请通过学术数据库检索真实研究。*
The MAP3K14 (Mitogen-Activated Protein Kinase Kinase Kinase 14) antibody is a crucial tool for studying the NF-κB signaling pathway. MAP3K14. also known as NIK (NF-κB-inducing kinase), is a serine/threonine kinase that activates the non-canonical NF-κB pathway by phosphorylating IKKα. This pathway regulates immune responses, B-cell survival, lymphoid organogenesis, and osteoclast differentiation. Dysregulation of MAP3K14 is linked to cancers, autoimmune disorders, and inflammatory diseases.
Antibodies targeting MAP3K14 are widely used in Western blotting, immunohistochemistry, immunoprecipitation, and flow cytometry to detect protein expression, localization, and activation status. They help identify MAP3K14's role in cellular processes and disease mechanisms. High-quality MAP3K14 antibodies exhibit specificity for distinct isoforms or phosphorylation states, validated via knockout controls or siRNA knockdown.
Research applications include investigating MAP3K14's interaction with TRAF proteins, its degradation via ubiquitination, and its crosstalk with other signaling pathways. Commercial antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal variants available. Proper validation ensures minimal cross-reactivity with related kinases (e.g., MAP3K1/2). As therapeutic targeting of NF-κB pathways advances, MAP3K14 antibodies remain vital for preclinical studies and biomarker discovery.
×
