| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RIP; RIP1; IMD57; RIP-1 |
| Entrez GeneID | 8737 |
| clone | 3B5B6 |
| WB Predicted band size | 76kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RIPK1 (AA: 131-277) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RIPK1抗体的3-4篇代表性文献的简要总结：
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1. **文献名称**：*Identification of RIP1 kinase as a specific cellular target of necrostatins*
**作者**：Degterev, A. et al.
**摘要**：该研究首次报道了RIPK1在坏死性凋亡(necroptosis)中的关键作用，并开发了特异性小分子抑制剂Necrostatin-1(通过靶向RIPK1激酶活性)。研究中利用抗RIPK1抗体验证了其在细胞死亡通路中的功能，为后续抗体工具的开发奠定了基础。
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2. **文献名称**：*RIPK1 inhibits ZBP1-driven necroptosis during development*
**作者**：Newton, K. et al.
**摘要**：通过基因工程小鼠模型和抗RIPK1抗体的应用，研究发现RIPK1的激酶活性在胚胎发育过程中抑制ZBP1介导的坏死性凋亡，揭示了其双重调控作用(促存活与促死亡)，并强调了RIPK1磷酸化修饰的功能重要性。
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3. **文献名称**：*RIPK1 ensures intestinal homeostasis by protecting the epithelium against apoptosis*
**作者**：Kaiser, W.J. et al.
**摘要**：利用RIPK1条件性敲除小鼠和特异性抗体，研究证明肠道上皮细胞中RIPK1通过抑制caspase-8依赖性凋亡维持屏障完整性。该研究为炎症性肠病等疾病中RIPK1的靶向治疗提供了依据。
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4. **文献名称**：*RIPK1-specific antibodies reveal novel activation mechanisms in inflammation and infection*
**作者**：Moujalled, D. et al.
**摘要**：该研究开发了高特异性抗RIPK1抗体，用于检测其寡聚化及翻译后修饰(如泛素化)，揭示了RIPK1在病原体感染和炎症反应中的动态调控机制，为疾病诊断和治疗提供了新工具。
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以上文献涵盖了RIPK1抗体的功能研究、工具开发及疾病关联，均发表于领域内高影响力期刊(如*Nature*、*Cell*等)，建议通过PubMed或Google Scholar获取全文。
Receptor-interacting protein kinase 1 (RIPK1) is a key regulator of cell survival, inflammation, and programmed cell death pathways, including apoptosis and necroptosis. As a serine/threonine kinase, RIPK1 interacts with death receptors (e.g., TNF receptor) and adaptor proteins to mediate downstream signaling. Depending on cellular context, it can promote NF-κB-mediated pro-survival signals or initiate death signaling complexes (e.g., necrosome). Dysregulation of RIPK1 is implicated in inflammatory diseases, neurodegenerative disorders, and cancer.
RIPK1 antibodies are essential tools for studying its expression, post-translational modifications (e.g., phosphorylation at Ser166), and interactions. They are widely used in techniques like Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry. Some antibodies target specific active forms (e.g., phosphorylated RIPK1) to assess kinase activity, while others detect total RIPK1 levels. Researchers often validate these antibodies using RIPK1-knockout cells or tissues to ensure specificity.
Therapeutic interest in RIPK1 has driven antibody development for preclinical studies, particularly in modulating inflammatory responses or cell death in conditions like Alzheimer’s disease, multiple sclerosis, and ischemic injury. Recent studies also explore RIPK1's role in immune cell regulation and its potential as a biomarker. However, interpreting results requires caution due to RIPK1's dual roles and context-dependent functions across cell types.
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