| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TR4; TAK1 |
| Entrez GeneID | 7182 |
| clone | 8H11D4 |
| WB Predicted band size | 65.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NR2C2 (AA: 62-356) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NR2C2抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
1. **"Characterization of a monoclonal antibody against human NR2C2 (TR4) for chromatin immunoprecipitation assays"**
- **作者**: Smith J et al.
- **摘要**: 本研究开发并验证了一种针对人NR2C2蛋白的单克隆抗体，证实其在染色质免疫沉淀(ChIP)中的特异性。通过Western blot和免疫荧光实验证明该抗体可特异性识别内源性NR2C2.并成功应用于前列腺癌细胞中NR2C2靶基因的鉴定。
2. **"NR2C2 modulates lipid metabolism through PPARγ interaction: Insights from antibody-based inhibition studies"**
- **作者**: Lee H et al.
- **摘要**: 利用NR2C2特异性抗体阻断其功能，揭示了NR2C2通过与PPARγ相互作用调控脂肪细胞分化和脂代谢的机制。抗体阻断实验表明NR2C2缺失导致脂质积累减少，提示其作为代谢性疾病的潜在治疗靶点。
3. **"Validation of NR2C2 antibody for immunohistochemical analysis in breast cancer tissues"**
- **作者**: Garcia R et al.
- **摘要**: 本文验证了一种商业化NR2C2抗体在乳腺癌组织免疫组化(IHC)中的应用，证实其与肿瘤分级和患者预后的相关性。实验显示NR2C2高表达与ER阴性乳腺癌的侵袭性相关。
(注：以上文献信息为示例性内容，实际文献需通过PubMed/Google Scholar等平台检索确认。)
The NR2C2 (Nuclear Receptor Subfamily 2 Group C Member 2) antibody is a tool used to study the TR4 orphan nuclear receptor, encoded by the *NR2C2* gene. TR4 belongs to the nuclear hormone receptor superfamily, which regulates gene expression by binding DNA response elements. Though classified as an "orphan" receptor due to unknown endogenous ligands, TR4 is implicated in diverse biological processes, including metabolism, embryonic development, and circadian rhythm regulation. It functions by forming homodimers or heterodimers with other nuclear receptors (e.g., RXR) to modulate target genes involved in lipid homeostasis, glucose metabolism, and cellular differentiation.
NR2C2 antibodies are essential for detecting TR4 protein expression, localization, and interaction partners in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. Research links TR4 to pathologies such as cancer (prostate, liver), metabolic syndrome, and neurodegenerative diseases, highlighting its dual roles in health and disease. For instance, TR4 may act as an oncogene by promoting tumor growth or as a tumor suppressor, depending on cellular context. Variants in *NR2C2* are also associated with insulin resistance and lipid disorders. However, its mechanisms remain partially unresolved, necessitating further study. Commercial NR2C2 antibodies are typically validated for specificity using knockout controls, ensuring reliable experimental outcomes in both basic and translational research.
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