| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RbAp46 |
| Entrez GeneID | 5931 |
| clone | 2A10C2 |
| WB Predicted band size | 47.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human RBBP7 (AA: 1-200) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RBBP7抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*RBBP7 interacts with the MTA1 subunit of the NuRD complex to promote breast cancer progression*
**作者**：Li, Y., et al.
**摘要**：本研究利用RBBP7抗体进行免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)，揭示了RBBP7通过与NuRD复合体中的MTA1相互作用，促进乳腺癌细胞的增殖和转移，并调控下游致癌基因的转录。
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2. **文献名称**：*Epigenetic regulation by RBBP7 in pluripotent stem cell maintenance*
**作者**：Smith, J., et al.
**摘要**：通过Western blot和免疫荧光技术使用RBBP7抗体，研究发现RBBP7在维持多能干细胞自我更新中起关键作用，其缺失导致组蛋白H3K27甲基化水平异常，进而影响多能性相关基因的表达。
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3. **文献名称**：*Development and validation of a specific RBBP7 monoclonal antibody for clinical diagnostics*
**作者**：Wang, H., et al.
**摘要**：该文献报道了一种新型RBBP7单克隆抗体的开发与验证，通过ELISA、免疫组化(IHC)和流式细胞术证实其高特异性和灵敏度，适用于卵巢癌组织样本中RBBP7蛋白表达的定量检测。
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**注**：以上文献为示例，实际引用时需核对数据库(如PubMed)获取准确信息。若需具体文献，可进一步提供研究方向(如癌症、表观遗传学等)以缩小范围。
**Background of RBBP7 Antibody**
RBBP7 (Retinoblastoma-Binding Protein 7), also known as RbAp46. is a member of the WD40 repeat protein family that plays a critical role in chromatin remodeling and epigenetic regulation. It interacts with histone deacetylases (HDACs) and polycomb group proteins, contributing to transcriptional repression and chromatin condensation. RBBP7 is a core component of several chromatin-modifying complexes, including the SIN3/HDAC, NuRD, and PRC2 complexes, which regulate gene expression during development, cell cycle progression, and differentiation.
Antibodies targeting RBBP7 are essential tools for studying its function in these processes. They are widely used in applications such as Western blotting, immunoprecipitation, and immunofluorescence to detect RBBP7 expression, localization, and protein-protein interactions. Research involving RBBP7 antibodies has linked the protein to cancer biology, as dysregulation of chromatin modifiers is common in malignancies. For example, RBBP7’s role in maintaining stem cell pluripotency and promoting tumorigenesis highlights its dual impact in normal physiology and disease.
These antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection. Both monoclonal and polyclonal RBBP7 antibodies are available, with selection depending on experimental needs. Understanding RBBP7’s regulatory mechanisms through such tools continues to advance insights into epigenetics, cancer therapy, and developmental biology.
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