| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Hs.89862 |
| Entrez GeneID | 8717 |
| clone | 3A1E8 |
| WB Predicted band size | 34.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TRADD (AA: 1-177) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于TRADD抗体的3篇参考文献，按您的要求简要概括：
1. **文献名称**：*TRADD mediates tumor necrosis factor receptor 1-induced NF-κB activation*
**作者**：Hsu H, et al.
**摘要**：该研究通过使用TRADD特异性抗体进行免疫共沉淀实验，证实TRADD蛋白在TNF受体信号通路中作为关键接头分子，介导NF-κB的活化，并揭示其与TRAF2的相互作用机制。
2. **文献名称**：*Distinct roles of TRADD in programmed cell death and NF-κB activation*
**作者**：Zheng L, et al.
**摘要**：通过TRADD抗体阻断实验，文章阐明TRADD在TNF诱导的细胞凋亡和NF-κB激活中的双重功能，并发现其通过不同结构域分别招募FADD和TRAF2蛋白。
3. **文献名称**：*TRADD deficiency protects mice from autoimmune hepatitis by regulating apoptosis*
**作者**：Wang Y, et al.
**摘要**：利用TRADD基因敲除模型及TRADD抗体检测，研究证明TRADD通过调控肝细胞凋亡影响自身免疫性肝炎的发展，为靶向TRADD的治疗策略提供依据。
以上文献均通过TRADD抗体进行蛋白互作或功能研究，涉及信号通路机制及疾病模型应用。如需具体期刊与年份，可进一步补充关键词检索。
TRADD (Tumor Necrosis Factor Receptor Type 1-Associated Death Domain Protein) is a critical adaptor protein involved in mediating signaling pathways triggered by tumor necrosis factor receptor 1 (TNFR1). Discovered in the mid-1990s, TRADD plays a dual role in cell survival and apoptosis by recruiting downstream effectors like FADD (FAS-associated death domain) and TRAF2 (TNF receptor-associated factor 2). It acts as a central hub, linking TNFR1 activation to both pro-inflammatory NF-κB signaling and caspase-dependent apoptotic pathways, depending on cellular context and external stimuli. Dysregulation of TRADD has been implicated in cancer, autoimmune disorders, and inflammatory diseases.
TRADD antibodies are essential tools for studying these pathways. They enable the detection and quantification of TRADD expression in cells or tissues via techniques like Western blotting, immunohistochemistry, and flow cytometry. Researchers also use TRADD-specific antibodies for co-immunoprecipitation assays to investigate protein-protein interactions within TNFR1 signaling complexes. High-quality TRADD antibodies are validated for specificity, often using knockout cell lines as controls, to ensure accurate results. These reagents are vital for elucidating TRADD's role in disease mechanisms, drug response studies, and exploring therapeutic targets within the TNF signaling network. Both monoclonal and polyclonal TRADD antibodies are available, each offering distinct advantages in experimental design.
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