| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Lobe; PRAS40 |
| Entrez GeneID | 84335 |
| clone | 6H1C6 |
| WB Predicted band size | 27.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human AKT1S1 (AA: 92-276) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于AKT1S1抗体的3篇参考文献的简要概括：
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1. **文献名称**：*mTORC1 signaling through PRAS40-regulated substrate phosphorylation*
**作者**：Sancak, Y. et al.
**摘要**：该研究通过使用AKT1S1(PRAS40)特异性抗体，揭示了其在mTORC1复合体中的调控作用。实验表明，PRAS40通过磷酸化状态影响底物结合，抗体用于免疫沉淀和Western blot以验证其与mTORC1的相互作用。
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2. **文献名称**：*AKT1S1 expression correlates with breast cancer progression and mTOR inhibition sensitivity*
**作者**：Hahn, K. et al.
**摘要**：研究利用AKT1S1抗体进行免疫组化和Western blot分析，发现AKT1S1在乳腺癌组织中高表达，且与mTOR抑制剂疗效相关，提示其作为潜在生物标志物的价值。
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3. **文献名称**：*Phosphorylation-dependent regulation of AKT1S1 in insulin signaling*
**作者**：Harrington, L.S. et al.
**摘要**：通过AKT1S1磷酸化特异性抗体，研究发现胰岛素刺激下AKT1S1的Ser183位点磷酸化增强，调控其与mTOR的结合，影响下游代谢通路，抗体用于检测磷酸化水平变化。
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以上文献均通过AKT1S1抗体探究其在信号通路、疾病机制或治疗中的应用。
The AKT1S1 antibody is a key tool for studying the AKT1 substrate 1 (AKT1S1), also known as PRAS40 (Proline-Rich AKT1 Substrate 1). AKT1S1 is a regulatory protein involved in the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway, which controls cell growth, proliferation, and metabolism. It acts as a scaffold linking mTORC1 to its downstream targets and is phosphorylated by AKT, enabling mTORC1 activation. Dysregulation of AKT1S1 is associated with cancers, metabolic disorders, and neurodegenerative diseases, making it a focus in biomedical research.
The AKT1S1 antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect AKT1S1 expression, post-translational modifications (e.g., phosphorylation at Thr246), and interactions with mTORC1 or other partners. Researchers rely on its specificity to distinguish AKT1S1 from homologous proteins and to study its role in cellular processes such as autophagy, apoptosis, and nutrient sensing. Commercial AKT1S1 antibodies are typically validated in multiple models, including human, mouse, and rat tissues. Understanding AKT1S1 dynamics through these antibodies provides insights into disease mechanisms and therapeutic targeting of the mTOR pathway.
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