| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Lobe; PRAS40 |
| Entrez GeneID | 84335 |
| clone | 4A11G7 |
| WB Predicted band size | 27.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human AKT1S1 (AA: 92-276) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于AKT1S1抗体的3篇参考文献及其摘要概述：
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1. **文献名称**：*PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding*
**作者**：Sancak, Y. et al.
**摘要**：该研究揭示了AKT1S1(PRAS40)通过直接结合mTORC1抑制其底物接入，调控细胞生长。研究使用AKT1S1抗体进行免疫沉淀和Western blot分析，证明PRAS40的磷酸化状态影响mTORC1活性及肿瘤发生。
2. **文献名称**：*Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40*
**作者**：Vander Haar, E. et al.
**摘要**：文章阐明了AKT1S1在胰岛素/mTOR信号通路中的作用，通过抗体检测发现PRAS40的AKT依赖性磷酸化解除其对mTORC1的抑制，从而促进营养吸收和细胞增殖。
3. **文献名称**：*PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase*
**作者**：Wang, L. et al.
**摘要**：该研究利用AKT1S1抗体验证PRAS40在代谢疾病中的调控机制，表明其磷酸化水平异常与胰岛素抵抗相关，为mTOR靶向治疗提供了新依据。
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以上文献均通过AKT1S1抗体探究其在mTOR通路、癌症及代谢疾病中的功能，涵盖分子机制与潜在临床应用。
The AKT1S1 antibody targets AKT1 substrate 1 (AKT1S1), also known as PRAS40 (proline-rich AKT1 substrate 1), a key regulatory protein in the mTORC1 (mechanistic target of rapamycin complex 1) signaling pathway. AKT1S1 interacts with mTORC1 as a component that modulates its activity in response to cellular nutrient and energy status. Phosphorylation of AKT1S1 by AKT at Thr246 promotes its dissociation from mTORC1. relieving inhibitory effects and enabling mTORC1 to drive processes like protein synthesis, cell growth, and metabolism. Dysregulation of this pathway is linked to cancers, metabolic disorders, and neurodegenerative diseases.
Antibodies against AKT1S1 are widely used in research to study its expression, localization, and post-translational modifications. They enable detection via techniques like Western blotting, immunoprecipitation, and immunohistochemistry. Phospho-specific antibodies targeting Thr246 are particularly valuable for assessing AKT-mediated regulatory events. AKT1S1 antibodies also help explore connections between mTORC1 signaling and conditions such as insulin resistance or tumor progression. Their applications extend to drug development, especially in evaluating inhibitors targeting AKT/mTOR pathways. As a biomarker, AKT1S1 expression or phosphorylation status may reflect pathway activation in disease models or therapeutic contexts.
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