| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PKBB; PRKBB; PKBBETA; RAC-BETA |
| Entrez GeneID | 208 |
| clone | 1B6 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human AKT2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ATG4B抗体的3篇参考文献示例(文献信息为虚构示例，仅用于演示格式)：
1. **文献名称**：ATG4B regulates autophagic flux and suppresses tumorigenesis through LC3 processing
**作者**：Li X. et al.
**摘要**：该研究利用ATG4B特异性抗体验证其在多种癌细胞系中的表达，发现抑制ATG4B活性可通过阻断LC3脂化抑制自噬，进而抑制肿瘤生长，提示其作为癌症治疗靶点的潜力。
2. **文献名称**：Development of a monoclonal antibody targeting ATG4B for colorectal cancer diagnosis
**作者**：Zhang Y. et al.
**摘要**：研究团队开发了高特异性人源化ATG4B单克隆抗体，通过免疫组化分析发现ATG4B在结直肠癌组织中显著高表达，且与患者预后不良相关，证明其作为生物标志物的价值。
3. **文献名称**：Structural insights into ATG4B protease activity by cryo-EM and antibody mapping
**作者**：Wang H. et al.
**摘要**：结合冷冻电镜和ATG4B抗体表位定位，揭示了ATG4B与底物LC3的结合构象，发现其催化口袋的动态调控机制，为设计小分子抑制剂提供结构基础。
注：以上为模拟文献，实际引用请通过PubMed/Google Scholar检索真实论文(可搜索关键词：ATG4B antibody, autophagy, cancer)。
ATG4B (Autophagy-related protein 4 homolog B) is a cysteine protease critical for autophagy, a cellular degradation process. It facilitates the cleavage of LC3 (Microtubule-associated proteins 1A/1B light chain 3) precursors to generate LC3-I, which is subsequently conjugated to phosphatidylethanolamine (forming LC3-II) to promote autophagosome membrane expansion. ATG4B also mediates LC3-II delipidation, recycling it for reuse in autophagy. Dysregulation of ATG4B is linked to cancer, neurodegenerative diseases, and metabolic disorders, making it a research target for therapeutic interventions.
ATG4B antibodies are essential tools for studying autophagy mechanisms. They enable detection of ATG4B expression levels, post-translational modifications, and subcellular localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use these antibodies to investigate ATG4B's regulatory roles in physiological and pathological conditions, such as its interaction with autophagy inhibitors/activators or its involvement in tumor progression. Commercial ATG4B antibodies are typically validated for specificity against human, mouse, or rat samples. However, batch variability and cross-reactivity with homologous proteins (e.g., ATG4A/C/D) require careful experimental controls. Recent studies also explore ATG4B inhibitors as potential anticancer agents, heightening the antibody's utility in drug development research.
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