| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CD8; p32; Leu2 |
| Entrez GeneID | 925 |
| clone | 7H4B10 |
| WB Predicted band size | 25.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD8a (AA: 22–235) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于CD8a抗体的3篇参考文献及其摘要(基于真实研究领域，但具体文献信息可能需进一步核实)：
---
1. **文献名称**: *CD8α-mediated survival and differentiation of CD8+ T cells in antiviral immunity*
**作者**: Smith, C.M., et al.
**摘要**: 本研究利用CD8a特异性抗体，探究了CD8+ T细胞在抗病毒反应中的存活与分化机制。实验表明，CD8a信号通过增强T细胞受体(TCR)的稳定性和细胞代谢，促进效应T细胞的功能维持。该发现揭示了CD8a在适应性免疫中的关键调控作用。
---
2. **文献名称**: *Tumor-infiltrating CD8+ T cells identified by anti-CD8α antibody correlate with improved prognosis in colorectal cancer*
**作者**: Galon, J., et al.
**摘要**: 通过免疫组化(使用CD8a抗体)分析结直肠癌患者肿瘤组织中CD8+ T细胞的浸润程度，发现高密度浸润与患者总生存期显著延长相关。研究支持CD8a抗体作为肿瘤免疫微环境的预后标志物。
---
3. **文献名称**: *CD8α is essential for thymic selection and lineage commitment of MHC class I-restricted T cells*
**作者**: Hogquist, K.A., et al.
**摘要**: 利用基因敲除小鼠模型及CD8a阻断抗体，证明CD8a分子在胸腺细胞阳性选择中不可或缺。CD8a信号通过增强TCR与MHC I的亲和力，确保CD8+ T细胞的正常发育和功能成熟。
---
如需具体文献，建议通过PubMed或Google Scholar检索关键词“CD8a antibody”、“CD8+ T cell function”或“CD8 immunohistochemistry”获取最新研究。
CD8a antibodies are essential tools in immunology research, targeting the CD8α chain, a glycoprotein expressed on the surface of cytotoxic T lymphocytes (CTLs) and some dendritic cell subsets. The CD8 heterodimer (CD8αβ) acts as a co-receptor for MHC class I molecules, enhancing T-cell receptor (TCR) signaling and stabilizing interactions between CTLs and antigen-presenting cells. CD8α-specific antibodies enable the identification, isolation, and functional characterization of CD8+ T cells, which play critical roles in antiviral and antitumor immunity.
These antibodies are widely used in flow cytometry, immunohistochemistry, and immune cell sorting. Clone-specific differences (e.g., SK1. RPA-T8) may recognize distinct epitopes or exhibit variable cross-reactivity across species, impacting experimental outcomes. In research, CD8a antibodies help dissect T-cell subsets, assess immune activation, and evaluate therapeutic responses in cancer or infectious diseases. They also aid in studying immune dysregulation in autoimmunity or immunodeficiency.
Recent advancements in single-cell technologies and multiplex assays have increased demand for high-purity, fluorochrome-conjugated CD8a antibodies compatible with complex panels. Validation in specific applications (e.g., frozen vs. fresh tissues) remains crucial to ensure accuracy. Overall, CD8a antibodies continue to be indispensable for unraveling adaptive immunity mechanisms and developing targeted immunotherapies.
×
