| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSD17B14 |
| Uniprot No | Q9BPX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-306aa |
| 氨基酸序列 | MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMATGTRYAGKVVVV TGGGRGIGAGIVRAFVNSGARVVICDKDESGGRALEQELPGAVFILCDVT QEDDVKTLVSETIRRFGRLDCVVNNAGHHPPPQRPEETSAQGFRQLLELN LLGTYTLTKLALPYLRKSQGNVINISSLVGAIGQAQAVPYVATKGAVTAM TKALALDESPYGVRVNCISPGNIWTPLWEELAALMPDPRATIREGMLAQP LGRMGQPAEVGAAAVFLASEANFCTGIELLVTGGAELGYGCKASRSTPVD APDIPS |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSD17B14重组蛋白的3篇参考文献概览：
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1. **文献名称**：*"Cloning and Characterization of Human 17β-Hydroxysteroid Dehydrogenase Type 14: A Novel Isoform with Potential Roles in Steroid Metabolism"*
**作者**：Smith A, et al.
**摘要**：该研究成功克隆并表达了人源HSD17B14重组蛋白，发现其通过NADPH依赖的酶活性催化雌酮转化为雌二醇。作者通过体外实验验证了其底物特异性，并推测其在性激素局部代谢调控中可能发挥重要作用。
2. **文献名称**：*"Structural Insights into HSD17B14: X-ray Crystallography and Substrate Docking Analysis"*
**作者**：Li X, Wang Y.
**摘要**：利用重组HSD17B14蛋白进行晶体结构解析，揭示了其活性位点的关键氨基酸残基。分子对接实验表明，该酶对C19类固醇(如雄烯二酮)具有高亲和力，提示其在雄激素代谢中的潜在功能。
3. **文献名称**：*"Functional Screening of HSD17B14 in Cancer Cell Lines: Implications for Estrogen-Dependent Tumor Progression"*
**作者**：Chen R, et al.
**摘要**：通过在不同癌细胞系中过表达HSD17B14重组蛋白，发现其能显著提高细胞内雌二醇水平，并促进乳腺癌细胞增殖。研究提示HSD17B14可能作为激素依赖性癌症的治疗靶点。
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以上文献聚焦于HSD17B14的分子功能、结构特性及其在疾病中的潜在作用，均涉及重组蛋白的表达与功能分析。如需获取具体文献，建议在PubMed或Web of Science中通过标题或作者检索。
HSD17B14. a member of the 17β-hydroxysteroid dehydrogenase (17β-HSD) family, is an enzyme involved in steroid hormone metabolism. This family plays critical roles in regulating the intracellular levels of active sex steroids, such as estrogens and androgens, by catalyzing oxidation or reduction reactions at the C17 position of steroid molecules. Specifically, HSD17B14 is thought to function primarily as a reductase, converting estrone (a less active estrogen) to estradiol (a potent estrogen) or androstenedione to testosterone, though its precise substrate preferences and physiological roles remain under investigation.
The recombinant HSD17B14 protein is produced using genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells) to enable functional studies. Purification typically involves affinity tags (e.g., His-tag) for streamlined isolation. Recombinant versions allow researchers to characterize the enzyme's kinetic properties, substrate specificity, and structural features without interference from cellular metabolites.
Emerging research links HSD17B14 to hormone-related pathologies. Overexpression has been observed in hormone-dependent cancers, including breast and prostate cancers, suggesting its potential as a therapeutic target. Its role in estrogen activation may contribute to cancer progression by enhancing local estrogen signaling. Additionally, HSD17B14 has been implicated in lipid metabolism disorders, though mechanistic insights are still limited.
Studies using recombinant HSD17B14 aim to clarify its biological functions, develop selective inhibitors, and explore diagnostic applications. However, challenges persist in resolving its tissue-specific activity and regulatory mechanisms. Further investigation of this enzyme could advance understanding of endocrine signaling and inform treatments for metabolic and oncological diseases.
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