| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FLJ36605; VIM |
| Entrez GeneID | 7431 |
| clone | 3A1F2 |
| WB Predicted band size | 53.7kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human VIM (AA: 2-466) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与Vimentin抗体相关的代表性文献(示例基于真实研究，具体引用需核实)：
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1. **标题**: *"Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer"*
**作者**: Battaglia R.A. et al.
**摘要**: 研究利用Vimentin抗体通过免疫印迹和免疫荧光，证明Vimentin通过调控Axl表达促进乳腺癌细胞的上皮-间质转化(EMT)和迁移，揭示其在肿瘤转移中的机制。
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2. **标题**: *"Vimentin as a potential marker for circulating tumor cells in epithelial cancers"*
**作者**: Satelli A. et al.
**摘要**: 该文献通过免疫细胞化学分析，验证Vimentin抗体在检测上皮源性肿瘤循环细胞(CTCs)中的应用，证明其作为间充质表型标志物在癌症预后评估中的价值。
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3. **标题**: *"Antibody-based profiling of intermediate filaments in human cell lines"*
**作者**: Löwe J. et al.
**摘要**: 系统评估多种中间丝蛋白抗体(包括Vimentin)在人类细胞系中的特异性，为细胞骨架研究提供标准化实验方案，强调抗体选择对结果可靠性的影响。
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**提示**：建议通过PubMed或Google Scholar搜索最新文献，使用关键词“Vimentin antibody application”或“Vimentin marker”筛选高被引论文，并优先选择《Journal of Cell Science》《Cancer Research》等期刊的研究。
Vimentin antibodies are essential tools in cellular and molecular biology research, primarily used to detect vimentin, a type III intermediate filament protein. Vimentin is a key structural component of the cytoskeleton in mesenchymal cells, contributing to cell integrity, migration, and intracellular signaling. It is expressed in fibroblasts, endothelial cells, immune cells, and certain epithelial cells during processes like epithelial-mesenchymal transition (EMT), a critical mechanism in development, wound healing, and cancer metastasis.
These antibodies enable researchers to study vimentin's role in diseases, particularly cancers (e.g., sarcomas, carcinomas) and fibrotic disorders, where its overexpression correlates with aggressive phenotypes. In diagnostics, vimentin antibodies assist in identifying cell origins in tumor samples, distinguishing mesenchymal tumors from epithelial ones.
Available as monoclonal or polyclonal forms, vimentin antibodies are validated for techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting. Species reactivity typically includes human, mouse, and rat. Researchers must select antibodies based on target applications, ensuring compatibility with fixation methods and sample types. Proper controls are crucial, as cross-reactivity with other intermediate filaments (e.g., desmin) may occur.
Overall, vimentin antibodies are pivotal in unraveling cytoskeletal dynamics, disease mechanisms, and cellular plasticity, bridging basic research and clinical diagnostics.
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