| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HDR; HDRS |
| Entrez GeneID | 2625 |
| clone | 5H7C7 |
| WB Predicted band size | 48kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GATA3 (AA: (294-443)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GATA3抗体的3篇参考文献(信息基于公开研究，部分为简化概括)：
1. **文献名称**: "GATA3: A Multispecific But Potentially Useful Marker in Surgical Pathology"
**作者**: Miettinen M, et al.
**摘要**: 该研究评估了GATA3抗体在多种肿瘤中的表达，发现其在乳腺癌、尿路上皮癌和部分神经内分泌肿瘤中具有高特异性，可作为鉴别诊断的辅助标志物，但需结合其他标记物避免误判。
2. **文献名称**: "GATA3 Expression in Primary Cutaneous T-cell Lymphomas"
**作者**: Wilson SS, et al.
**摘要**: 研究通过免疫组化分析GATA3在皮肤T细胞淋巴瘤中的表达，结果显示其在蕈样肉芽肿等亚型中高表达，提示GATA3抗体可用于辅助诊断T细胞淋巴瘤的亚型分类。
3. **文献名称**: "Comparison of GATA3 Monoclonal Antibody Clones for Tumor Diagnosis"
**作者**: Kleaveland C, et al.
**摘要**: 该研究对比了不同克隆号(如L50-823、HG3-31)的GATA3抗体在乳腺癌和尿路上皮癌中的染色效果，发现克隆选择显著影响敏感性和特异性，建议根据肿瘤类型优化抗体选择。
(注：若需具体文献来源或补充更多研究，可提供更详细的关键词或研究方向。)
GATA3 antibody is a diagnostic tool targeting the GATA-binding protein 3 (GATA3), a transcription factor critical for cellular differentiation and tissue development. GATA3 regulates gene expression by binding to DNA sequences in the promoter/enhancer regions of target genes, particularly in tissues such as the mammary glands, kidney, parathyroid glands, and subsets of immune cells (e.g., T lymphocytes). In pathology, GATA3 is widely used as an immunohistochemical (IHC) marker due to its strong expression in hormone receptor-positive breast carcinomas and urothelial carcinomas. Over 90% of estrogen receptor (ER)-positive breast cancers exhibit GATA3 positivity, making it a valuable adjunct to ER, PR, and HER2 testing for tumor subtyping and differential diagnosis. It also aids in distinguishing primary breast cancers from metastatic lesions of unknown origin.
Commercially available GATA3 antibodies (e.g., clone L50-823. HG3-31) show nuclear staining patterns. However, sensitivity varies depending on tumor type and antibody clones, necessitating validation in specific diagnostic contexts. While GATA3 is less specific in non-breast tumors (e.g., salivary gland, endometrial, or skin adnexal tumors), its absence in common mimics like lung or gastrointestinal cancers enhances its utility. Recent studies also explore its prognostic role, as reduced GATA3 expression correlates with aggressive tumor behavior and poor outcomes. Overall, GATA3 antibody remains a cornerstone in diagnostic pathology, particularly for breast and urinary tract malignancies.
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