| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5J2 |
| Uniprot No | P56134 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-94aa |
| 氨基酸序列 | MASVGECPAPVPVKDKKLLEVKLGELPSWILMRDFSPSGIFGAFQRGYYRYYNKYINVKKGSISGITMVLACYVLFSYSFSYKHLKHERLRKYH |
| 预测分子量 | 12.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP5J2重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询验证)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of ATP5J2 in Mitochondrial ATP Synthase Assembly"*
**作者**: Chen L, Wang Y, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组ATP5J2蛋白，并验证其作为线粒体ATP合酶亚基的功能。通过体外重组实验，发现ATP5J2对复合物V的组装及质子通道活性具有关键作用，为线粒体能量代谢研究提供新工具。
2. **文献名称**: *"ATP5J2 Regulates Mitochondrial Respiratory Chain Stability via Interaction with Complex V"*
**作者**: Kim S, Park JH, et al.
**摘要**: 通过免疫共沉淀和基因敲除技术，揭示ATP5J2重组蛋白与线粒体复合物V的相互作用机制，表明其缺失会导致呼吸链功能障碍及ROS积累，提示其在氧化应激相关疾病中的潜在意义。
3. **文献名称**: *"Structural Analysis of ATP5J2 Using Cryo-EM Reveals Proton Transport Pathways"*
**作者**: González-Barroso MM, Morales-Ruiz M, et al.
**摘要**: 利用冷冻电镜技术解析ATP5J2重组蛋白的高分辨率结构，阐明其在线粒体膜质子梯度形成中的构象变化，为靶向ATP合酶的药物设计提供结构基础。
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如需获取真实文献，建议通过PubMed、Web of Science等平台搜索“ATP5J2 recombinant protein”或相关关键词。
ATP5J2. also known as ATP synthase membrane subunit d, is a key component of the mitochondrial ATP synthase complex (Complex V), which plays a central role in cellular energy production. This enzyme catalyzes the final step of oxidative phosphorylation by synthesizing adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate, utilizing the proton gradient across the inner mitochondrial membrane. The ATP synthase complex consists of two main structural domains: the membrane-embedded F0 sector, responsible for proton translocation, and the catalytic F1 sector, which drives ATP synthesis. ATP5J2 is a subunit of the F0 domain, encoded by the nuclear gene ATP5J2 located on chromosome 5 in humans. It contributes to the stability and assembly of the F0 proton channel, facilitating efficient coupling of proton flow with ATP production.
Recombinant ATP5J2 protein is produced through heterologous expression systems, such as *E. coli* or mammalian cell lines, enabling studies on its structural and functional properties. Its recombinant form often includes affinity tags (e.g., His-tag) to simplify purification. Researchers utilize this protein to investigate mitochondrial disorders linked to ATP synthase dysfunction, including neurodegenerative diseases, cardiomyopathies, and metabolic syndromes. Additionally, ATP5J2 serves as a tool for exploring the molecular mechanisms of ATP synthase assembly, proton transport regulation, and energy metabolism. Its role in cellular bioenergetics also makes it relevant in cancer research, as altered mitochondrial function is a hallmark of tumorigenesis. By studying recombinant ATP5J2. scientists aim to develop targeted therapies for diseases associated with mitochondrial energy deficits.
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