| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGJ |
| Uniprot No | P01591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-159aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSQEDERIVLVDNKCKCARITSRIIRSSE DPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEV ELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVET ALTPDACYPD |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于IGJ(免疫球蛋白J链)重组蛋白研究的假设性参考文献示例，供参考：
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1. **文献名称**：Expression and Purification of Recombinant Human Immunoglobulin J Chain in Escherichia coli
**作者**：Smith A, et al.
**摘要**：研究报道了在大肠杆菌中高效表达重组人源IGJ蛋白的优化方法，通过His标签纯化获得高纯度产物，并验证其促进IgA多聚化的功能活性。
2. **文献名称**：Structural Insights into J Chain-Mediated Polymerization of Immunoglobulin A
**作者**：Lee J, et al.
**摘要**：通过X射线晶体学解析IGJ与IgA结合的结构，揭示IGJ通过二硫键介导多聚IgA组装的关键机制，为黏膜抗体工程设计提供依据。
3. **文献名称**：Role of Recombinant J Chain in Enhancing Mucosal Immunity through Polymeric IgM Assembly
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞表达重组IGJ，证实其促进IgM五聚体形成的能力，并显著增强小鼠模型中的黏膜免疫应答。
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**说明**：以上文献为假设示例，实际研究中建议通过PubMed、Google Scholar等平台检索关键词如“recombinant immunoglobulin J chain”、“J chain protein engineering”获取真实文献。
**Background of IGJ Recombinant Protein**
The IGJ (Immunoglobulin J-chain) protein is a small, cysteine-rich polypeptide critical for the assembly and function of polymeric immunoglobulins (Igs), particularly IgA and IgM. It facilitates the polymerization of antibody subunits by linking monomeric Ig units via disulfide bonds, enabling the formation of dimeric IgA (dIgA) and pentameric IgM. These multimeric antibodies play essential roles in mucosal immunity and systemic immune responses, as they can efficiently neutralize pathogens, agglutinate antigens, and activate complement pathways.
Recombinant IGJ protein is produced using biotechnological methods, typically through the expression of the *IGJ* gene in heterologous systems such as *E. coli*, yeast, or mammalian cell lines. Mammalian systems are often preferred due to their ability to perform post-translational modifications, ensuring proper folding and functionality. The recombinant protein is purified via affinity chromatography, leveraging tags like His-tag or Fc-fusion constructs for streamlined isolation.
Research on IGJ recombinant protein has gained momentum due to its therapeutic and diagnostic applications. It is used to study the structural and functional properties of polymeric antibodies, aiding in the development of mucosal vaccines, antibody-based therapies, and diagnostics targeting pathogens like viruses and bacteria. Additionally, IGJ is explored in autoimmune disease research, as dysregulated polymeric antibody production is linked to conditions such as lupus and rheumatoid arthritis.
Despite its significance, challenges persist in optimizing IGJ expression yields and stability, particularly in large-scale production. Advances in protein engineering and expression systems continue to address these limitations, enhancing its utility in both basic research and clinical applications. Overall, IGJ recombinant protein serves as a vital tool for understanding immune mechanisms and advancing immunotherapeutic strategies.
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