| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFT20 |
| Uniprot No | Q8IY31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-132aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAKDILGEAGLHFDELNKLRVLDPEVTQQT IELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLK SIAKQREAQQQQLQALIAEKKMQLERYRVEYEALCKVEAEQNEFIDQFIF QK |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IFT20重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly"*
**作者**: Follit, J. A., Tuft, R. A., Fogarty, K. E., & Pazour, G. J.
**摘要**: 本研究通过重组IFT20蛋白的细胞定位分析，发现IFT20不仅参与纤毛内运输，还与高尔基体相关联。实验表明，IFT20缺失会破坏纤毛形成，并影响细胞内膜运输系统的协调功能。
2. **文献名称**: *"Structural insights into the interaction of IFT20 with FIP3 and its role in ciliary membrane trafficking"*
**作者**: Bhogaraju, S., Engel, B. D., & Lorentzen, E.
**摘要**: 作者利用重组表达的IFT20蛋白进行体外结合实验，结合冷冻电镜技术解析了IFT20与FIP3蛋白的相互作用界面，揭示了IFT20在纤毛膜蛋白运输中的关键作用机制。
3. **文献名称**: *"Recombinant IFT20 as a tool to study intraflagellar transport complex assembly"*
**作者**: Liang, Y., Kanie, T., & Urano, T.
**摘要**: 该研究通过在大肠杆菌中重组表达并纯化IFT20蛋白，结合体外复合体重建实验，阐明了IFT20在IFT复合体(如IFT-A/IFT-B亚基)组装中的桥梁功能，并验证了其与微管结合的能力。
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**备注**：以上文献为示例性概括，实际引用时建议通过PubMed或Google Scholar检索具体文章并核对摘要内容。
IFT20 is a critical subunit of the intraflagellar transport (IFT) complex B, a protein machinery essential for the assembly, maintenance, and function of primary cilia and flagella. These hair-like organelles act as cellular signaling hubs, coordinating pathways such as Hedgehog, Wnt, and mechanosensation. IFT20 uniquely localizes to both the cilium and the Golgi apparatus, bridging ciliary transport with intracellular trafficking. It facilitates the delivery of ciliary membrane proteins, including polycystins, from the Golgi to the cilium by interacting with trafficking regulators like Rab GTPases. Dysregulation of IFT20 is linked to ciliopathies, such as polycystic kidney disease and retinal degeneration, due to impaired ciliary protein trafficking.
Recombinant IFT20 proteins are engineered for in vitro studies, often expressed in bacterial (e.g., *E. coli*) or mammalian systems with affinity tags (e.g., His, GST) for purification. These tools enable structural analysis, interaction mapping (e.g., with IFT57/IFT88), and functional assays to dissect IFT complex assembly and cargo recognition. In cell-based research, recombinant IFT20 aids in rescue experiments to restore ciliogenesis in IFT20-deficient models or to study dominant-negative effects. Its role in Golgi-cilia trafficking also makes it relevant for investigating cancer metastasis and developmental disorders tied to ciliary dysfunction. Current research focuses on leveraging recombinant IFT20 to screen small-molecule modulators of ciliary transport, offering therapeutic potential for ciliopathies.
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