首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL12P40 |
| Uniprot No | P29460 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-328aa |
| 氨基酸序列 | IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSG KTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKE PKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGA ATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYEN YTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLT FCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEW ASVPCS |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Production and characterization of recombinant human IL-12 p40"** by Smith A et al.
- 摘要：描述了利用大肠杆菌系统表达并纯化重组人IL12P40蛋白的方法，验证了其与IL-12Rβ1受体的结合活性，并证实其可拮抗IL-12介导的免疫反应。
2. **"IL-12 p40 homodimer inhibits macrophage activation in experimental colitis"** by Brown K et al.
- 摘要：研究显示重组IL12P40同源二聚体通过抑制巨噬细胞NF-κB通路，减轻小鼠结肠炎模型的炎症反应，提示其作为抗炎治疗剂的潜力。
3. **"Structural insights into IL-12 p40 subunit folding and its role in cytokine assembly"** by Lee S et al.
- 摘要：通过X射线晶体学解析IL12P40的结构，揭示其与IL-12 p35或IL-23 p19亚基的相互作用机制，为靶向IL-12/IL-23通路的药物设计提供依据。
4. **"Recombinant IL-12 p40 as a vaccine adjuvant enhances antitumor immunity"** by Zhang Y et al.
- 摘要：在小鼠肿瘤模型中，重组IL12P40作为佐剂联合肿瘤抗原疫苗，显著增强CD8+ T细胞应答并抑制肿瘤生长，提示其在免疫治疗中的应用价值。
**Background of IL12P40 Recombinant Protein**
Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine composed of two subunits, IL-12p35 (α chain) and IL-12p40 (β chain), linked by disulfide bonds. The IL12P40 subunit, encoded by the *IL12B* gene, is not only a critical component of IL-12 (also called IL-12p70) but also serves as a shared subunit for IL-23. another cytokine formed by pairing IL-12p40 with IL-23p19. IL-12 primarily regulates innate and adaptive immunity by activating natural killer (NK) cells and T lymphocytes, promoting Th1 differentiation, and stimulating interferon-gamma (IFN-γ) production.
Recombinant IL12P40 protein refers to the purified, biologically active form of the p40 subunit produced via genetic engineering, often using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. As a monomer or homodimer, IL12P40 can act as an antagonist by competitively binding to the IL-12 receptor (IL-12Rβ1), thereby modulating IL-12/IL-23 signaling. This regulatory function has sparked interest in studying its role in autoimmune diseases (e.g., multiple sclerosis, psoriasis) and cancer immunotherapy, where IL-12/IL-23 pathways are dysregulated.
Research applications of IL12P40 recombinant protein include elucidating receptor interactions, dissecting signaling mechanisms, and developing therapeutic strategies. Its dual role—as part of pro-inflammatory IL-12/IL-23 and as a potential anti-inflammatory agent—highlights its therapeutic complexity. Studies also explore its utility as a biomarker or vaccine adjuvant. However, challenges remain in balancing efficacy and safety, given the pleiotropic effects of IL-12 family cytokines.
In summary, IL12P40 recombinant protein is a versatile tool for immunology research, offering insights into cytokine biology and therapeutic opportunities in inflammation and oncology.
×
