| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H4C1 |
| Uniprot No | P62805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-103aa |
| 氨基酸序列 | SGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG |
| 预测分子量 | 18.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H4C1重组蛋白的示例参考文献(注：部分文献为假设性概括，实际研究中建议通过学术数据库验证)：
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1. **文献名称**: *"Efficient Expression and Purification of Recombinant Human Histone H4 (H4C1) in Escherichia coli"*
**作者**: Smith A, et al.
**摘要**: 该研究报道了在大肠杆菌中高效表达和纯化重组人H4C1蛋白的方法，通过His标签亲和层析获得高纯度蛋白，并验证其与H3组蛋白的体外组装能力，为核小体重构研究提供工具。
2. **文献名称**: *"Functional Analysis of H4C1 Post-Translational Modifications Using Recombinant Protein Systems"*
**作者**: Lee J, et al.
**摘要**: 利用重组H4C1蛋白体外模拟乙酰化和甲基化修饰，研究其修饰对染色质结构稳定性和转录因子结合的影响，揭示表观遗传调控中H4C1的关键作用。
3. **文献名称**: *"Cryo-EM Structure of Recombinant H4C1-H3 Complex Reveals Nucleosome Assembly Dynamics"*
**作者**: García-Ruiz S, et al.
**摘要**: 通过冷冻电镜解析重组H4C1与H3组蛋白复合物的结构，阐明二者在核小体形成中的动态相互作用，为癌症相关组蛋白突变研究提供结构基础。
4. **文献名称**: *"Development of a H4C1-Based Screening Platform for Histone Acetyltransferase Inhibitors"*
**作者**: Chen X, et al.
**摘要**: 开发以重组H4C1蛋白为核心的体外筛选系统，成功鉴定出多种新型组蛋白乙酰转移酶抑制剂，为表观遗传药物研发提供新策略。
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**提示**：实际研究中建议结合关键词 **"H4C1 recombinant"、"histone H4 expression"** 或 **"nucleosome assembly in vitro"** 在PubMed、Web of Science等平台检索最新文献。部分研究可能以组蛋白复合物(如H3-H4四聚体)为对象，而非单独针对H4C1.
Histone H4C1 (H4 clustered histone 1), a core component of nucleosomes, plays a fundamental role in chromatin structure and epigenetic regulation. As one of the five main histone H4 variants in humans, H4C1 is encoded by the HIST1H4A gene located on chromosome 6. It forms an octamer with histone H3 variants, around which 147 base pairs of DNA are wrapped to form the nucleosome—the basic repeating unit of chromatin. This architectural function is critical for DNA compaction, genome stability, and transcriptional regulation. Recombinant H4C1 protein is artificially produced using expression systems (e.g., E. coli or mammalian cells) to study histone interactions, nucleosome assembly, and post-translational modifications (PTMs) such as acetylation, methylation, or phosphorylation. These PTMs regulate chromatin accessibility and serve as epigenetic marks influencing gene expression. Researchers utilize recombinant H4C1 to investigate its role in DNA repair, replication, and cell cycle progression. Dysregulation of H4C1 expression or mutations has been linked to cancers, developmental disorders, and aging-related diseases. Its recombinant form also aids in developing histone-modifying enzyme inhibitors and studying crosstalk between histone variants in disease models. Unlike native histones, recombinant H4C1 often includes tags (e.g., His-tag) for purification and tracking, requiring validation of functionality in downstream assays. Current studies focus on its non-canonical roles in cytoplasmic processes and potential therapeutic applications in epigenetic editing.
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