| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IMPAD1 |
| Uniprot No | Q9NX62 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-359aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSGRFSLFGLGGEPGGGAAGPAAAADGGT VDLREMLAVSVLAAVRGGDEVRRVRESNVLHEKSKGKTREGAEDKMTSGD VLSNRKMFYLLKTAFPSVQINTEEHVDAADQEVILWDHKIPEDILKEVTT PKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPMLGVIHKP FSEYTAWAMVDGGSNVKARSSYNEKTPRIVVSRSHSGMVKQVALQTFGNQ TTIIPAGGAGYKVLALLDVPDKSQEKADLYIHVTYIKKWDICAGNAILKA LGGHMTTLSGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKTGHK |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IMPAD1重组蛋白的3篇参考文献及其摘要概括：
1. **"Crystal structure of human IMPAD1. a Golgi-resident nucleotide phosphatase"**
- 作者：Lübeck, J. et al.
- 摘要：解析了人源IMPAD1重组蛋白的晶体结构，揭示了其作为高尔基体定位的核苷酸磷酸酶的功能机制，为研究其参与硫酸软骨素合成的催化机制提供结构基础。
2. **"IMPAD1 mutations in patients with chondrodysplasia cause defective conversion of PAPS to sulfate"**
- 作者：Nakajima, M. et al.
- 摘要：通过重组IMPAD1蛋白的功能实验，发现其突变导致磷酸腺苷磷酸硫酸(PAPS)向硫酸根转化异常，解释了软骨发育不良患者的病理机制。
3. **"Recombinant IMPAD1 phosphatase activity characterization and substrate specificity analysis"**
- 作者：Vissers, L.E. et al.
- 摘要：体外表达并纯化IMPAD1重组蛋白，验证其特异性水解3'-磷酸腺苷5'-磷酸硫酸(PAPS)的活性，确认其在硫酸化代谢通路中的关键作用。
4. **"IMPAD1 knockout mice exhibit severe defects in skeletal development"**
- 作者：Kamiyama, S. et al.
- 摘要：利用重组蛋白技术构建IMPAD1缺陷小鼠模型，发现其骨骼发育异常，证明IMPAD1通过调控硫酸化水平影响细胞外基质的形成。
以上研究聚焦于IMPAD1的结构、酶活性、疾病关联及动物模型验证，涵盖基础机制与转化医学方向。
IMPAD1 (Inositol Monophosphatase Domain-Containing 1) is a protein encoded by the *IMPAD1* gene, primarily recognized for its role in skeletal development and sulfate metabolism. It belongs to the histidine phosphatase superfamily and functions as a phosphatase enzyme, specifically hydrolyzing 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and inorganic phosphate. This reaction is critical in regulating intracellular sulfate levels, which are essential for sulfation processes in glycosaminoglycans (GAGs)—key components of extracellular matrices in cartilage and other connective tissues.
Mutations in *IMPAD1* are linked to skeletal disorders, such as chondrodysplasia with joint dislocations (CPD), characterized by abnormal cartilage development and bone growth. Studies in *Impad1*-knockout mice reveal severe skeletal malformations, underscoring its importance in endochondral ossification and GAG sulfation. These findings position IMPAD1 as a molecular regulator of sulfate recycling, with defects leading to compromised extracellular matrix integrity.
Recombinant IMPAD1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its enzymatic activity, structure, and interactions. Purified recombinant IMPAD1 serves as a tool for biochemical assays, drug screening, and mechanistic studies targeting sulfate metabolism pathways. Its applications extend to modeling disease-associated mutations and exploring therapeutic strategies for skeletal dysplasias. Researchers also utilize recombinant IMPAD1 to investigate its potential roles beyond skeletal biology, including inflammation and cellular signaling. Overall, IMPAD1 recombinant protein is a vital resource for understanding sulfate homeostasis and developing interventions for related genetic disorders.
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