| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IMP3 |
| Uniprot No | Q9NV31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-184aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMVRKLKF HEQKLLKQVD FLNWEVTDHN LHELRVLRRY RLQRREDYTR YNQLSRAVRE LARRLRDLPE RDQFRVRASA ALLDKLYALG LVPTRGSLEL CDFVTASSFC RRRLPTVLLK LRMAQHLQAA VAFVEQGHVR VGPDVVTDPA FLVTRSMEDF VTWVDSSKIK RHVLEYNEER DDFDLEA |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IMP3重组蛋白的3篇参考文献概览，涵盖其功能、应用及结构研究：
1. **文献名称**：*IMP3 Recombinant Protein Enhances Pancreatic Cancer Cell Invasion through RNA Stabilization*
**作者**：Smith J, et al.
**摘要**：本研究利用大肠杆菌表达系统制备重组IMP3蛋白，发现其通过稳定特定mRNAs(如MMP9)促进胰腺癌细胞侵袭，为靶向IMP3的癌症治疗提供依据。
2. **文献名称**：*Structural Characterization of Recombinant IMP3 Using Cryo-Electron Microscopy*
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析IMP3重组蛋白的三维结构，揭示其KH结构域与RNA结合的分子机制，助力设计抑制IMP3-RNA相互作用的化合物。
3. **文献名称**：*Diagnostic Potential of Serum IMP3 Recombinant Protein in Ovarian Cancer*
**作者**：Wang Y, et al.
**摘要**：基于重组IMP3蛋白开发ELISA检测法，证实其在卵巢癌患者血清中显著高表达，敏感性达85%，支持其作为非侵入性诊断标志物的潜力。
*注：以上为模拟示例，实际文献需通过学术数据库检索确认。*
IMP3. also known as insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3), is a member of the conserved IMP family of RNA-binding proteins involved in post-transcriptional gene regulation. It was initially identified for its role in embryonic development, where it stabilizes and transports target mRNAs, including those encoding growth factors like IGF2. and regulates their translation. IMP3 is highly expressed during embryogenesis but shows limited expression in most adult tissues. However, its re-expression is frequently observed in various cancers, linking it to tumor progression and aggressiveness.
Structurally, IMP3 contains two RNA recognition motifs (RRMs) and four hnRNP K homology (KH) domains, enabling interactions with specific mRNA sequences. By binding to transcripts, IMP3 modulates their stability, localization, and protein synthesis, influencing critical cellular processes such as proliferation, migration, and invasion. For example, it enhances oncogenic pathways by stabilizing mRNAs encoding c-Myc, CD44. and MMP9.
In pathology, IMP3 overexpression is associated with poor prognosis in cancers like pancreatic, ovarian, and hepatocellular carcinoma. Its role in promoting metastasis and therapy resistance has made it a biomarker for tumor aggressiveness and a potential therapeutic target. Additionally, IMP3 autoantibodies have been detected in patient sera, suggesting diagnostic utility.
Recombinant IMP3 protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems. The purified protein retains RNA-binding activity and is used to study its molecular interactions, develop diagnostic assays, or generate antibodies. Researchers also employ IMP3 recombinant proteins to explore its functional mechanisms in cancer models and assess its potential as a therapeutic target. Its dual role in development and disease underscores its biological significance and translational relevance.
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