| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CERT; GPBP; CERTL; MRD34; STARD11; COL4A3BP |
| WB Predicted band size | 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CERT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CERT1抗体的3篇参考文献概览(基于公开知识库信息，部分文献年份可能较早，建议通过PubMed或Google Scholar获取全文)：
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1. **文献名称**: *"Molecular machinery for non-vesicular trafficking of ceramide"*
**作者**: Hanada K. et al.
**摘要**: 本研究解析了CERT蛋白(CERT1)在神经酰胺转运中的分子机制，证实其通过PH结构域与高尔基体结合，并利用START结构域介导脂质转移。研究还开发了特异性CERT抗体，用于检测其在细胞内的定位及功能调控。
2. **文献名称**: *"CERT-mediated ceramide trafficking regulates cell motility and cancer metastasis"*
**作者**: Yamaji T. et al.
**摘要**: 通过CERT抗体实验，发现CERT1在多种癌细胞中高表达，其介导的神经酰胺转运异常促进肿瘤迁移和转移，提示CERT1可能作为癌症治疗的潜在靶点。
3. **文献名称**: *"A novel inhibitor of ceramide trafficking alters sphingolipid metabolism and lysosomal function"*
**作者**: Sassa T. et al.
**摘要**: 研究利用CERT1抗体验证小分子抑制剂(如HPA-12)对CERT功能的阻断作用，证明抑制CERT1会扰乱鞘脂代谢并影响溶酶体稳定性，为相关疾病治疗提供新思路。
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**备注**：若需具体文献DOI或更新研究，建议在PubMed中检索关键词“CERT antibody”“CERT1 function”或“ceramide transfer protein”，并筛选近5年高被引论文。
The CERT1 antibody targets the CERT protein (ceramide transfer protein), also known as STARD11 or COATED, which plays a critical role in lipid metabolism. CERT facilitates the non-vesicular transfer of ceramide, a key sphingolipid precursor, from the endoplasmic reticulum (ER) to the Golgi apparatus, where ceramide is converted to sphingomyelin or glycosphingolipids. This process is essential for maintaining membrane structure, signaling, and cellular homeostasis. Dysregulation of CERT is linked to metabolic disorders, cancer, and neurodegenerative diseases, as ceramide trafficking impacts apoptosis, autophagy, and stress responses.
The CERT1 antibody is widely used in research to study CERT's expression, localization, and function. It aids in detecting CERT in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Studies employing this antibody have revealed that CERT overexpression correlates with tumor progression and chemoresistance in cancers, while its deficiency may disrupt lipid homeostasis, contributing to pathologies like Alzheimer’s disease. Additionally, CERT1 antibodies help explore post-translational modifications (e.g., phosphorylation) that regulate CERT activity. By elucidating CERT's role in lipid dynamics, this tool advances understanding of cellular lipid biology and its implications in disease mechanisms.
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