| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | CYP11A; CYPXIA1; P450SCC |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human CYP11A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP11A1抗体的3篇参考文献示例(注：内容为虚构，仅作演示用途)：
1. **文献名称**: "Immunolocalization of CYP11A1 in Human Adrenal Cortex and Its Role in Steroidogenesis"
**作者**: Miller WL, Auchus RJ
**摘要**: 本研究利用CYP11A1抗体通过免疫组化技术定位其在人肾上腺皮质细胞中的表达，证实其在线粒体中的分布，并探讨其在皮质醇和醛固酮合成中的关键作用。
2. **文献名称**: "Altered CYP11A1 Expression in Polycystic Ovary Syndrome Granulosa Cells"
**作者**: Sahmay S, Zhang X
**摘要**: 通过Western blot和免疫荧光分析，发现多囊卵巢综合征患者的卵巢颗粒细胞中CYP11A1蛋白表达显著降低，提示其与雌激素合成异常的相关性。
3. **文献名称**: "CYP11A1 Antibody Validation for Mitochondrial Protein Quantification in Aging Models"
**作者**: Pétervári E, Yoshioka H
**摘要**: 验证CYP11A1抗体的特异性及灵敏度，应用于衰老小鼠模型，发现肾上腺和睾丸中线粒体CYP11A1蛋白水平随年龄增长而下降，影响类固醇激素稳态。
4. **文献名称**: "CYP11A1 Mutations and Antibody-Based Detection in Congenital Adrenal Hyperplasia"
**作者**: Bose HS, Huang MC
**摘要**: 开发高特异性CYP11A1抗体用于先天性肾上腺皮质增生症患者的突变蛋白检测，揭示部分病例中酶功能缺陷与胆固醇代谢障碍的关联。
The CYP11A1 antibody is a crucial tool for studying the enzyme cytochrome P450 family 11 subfamily A member 1 (CYP11A1), also known as cholesterol side-chain cleavage enzyme (P450scc). This mitochondrial enzyme catalyzes the first and rate-limiting step in steroid hormone biosynthesis by converting cholesterol to pregnenolone. It is expressed primarily in steroidogenic tissues, including the adrenal glands, gonads, and placenta, making it essential for producing glucocorticoids, mineralocorticoids, and sex hormones.
CYP11A1 antibodies are widely used in research to detect and quantify the enzyme’s expression and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate disorders linked to steroidogenesis, such as congenital adrenal hyperplasia, adrenal insufficiency, and reproductive endocrine disorders. They also aid in exploring CYP11A1’s role in pathological conditions, including cancers where steroidogenic pathways are dysregulated.
Most CYP11A1 antibodies target specific epitopes, such as the N-terminal region or conserved catalytic domains, and are often raised in rabbits or mice. Validation includes testing on tissues or cell lines with known CYP11A1 expression. Researchers rely on these antibodies to study regulatory mechanisms of steroidogenesis, enzyme inhibition, and tissue-specific expression patterns, contributing to advancements in endocrinology, reproductive biology, and therapeutic development.
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