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Rabbit Polyclonal CRK Antibody

  • 中文名: CRK抗体
  • 别    名: p38; CRKII
货号: IPDX01236
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/2000-1/5000 Human,Mouse,Rat

产品详情

参考文献

以下是关于CRK抗体的模拟参考文献示例(请注意文献信息为虚构,仅供格式参考):

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1. **文献名称**: "CRK adaptor proteins regulate cell migration and invasion in cancer through antibody-mediated detection"

**作者**: Smith J, Lee K, et al.

**摘要**: 本研究利用CRK特异性抗体检测了多种癌细胞系中CRK-I/II亚型的表达差异,发现CRK蛋白的过表达与肿瘤细胞迁移能力增强相关。通过抗体阻断实验,证实CRK通过调控Rac1信号通路促进侵袭性表型。

2. **文献名称**: "Development and validation of a high-affinity monoclonal antibody for CRK phospho-specific analysis"

**作者**: Tanaka M, Yamaguchi T.

**摘要**: 研究团队开发了一种针对CRK磷酸化位点(Tyr221)的单克隆抗体,验证了其在Western blot和免疫荧光中的特异性。该抗体成功应用于研究EGFR信号激活下CRK的磷酸化动态及其在凋亡抵抗中的作用。

3. **文献名称**: "CRK antibody-based profiling identifies novel biomarkers in lung adenocarcinoma"

**作者**: Chen L, Wang H, et al.

**摘要**: 通过CRK抗体对临床肺癌组织样本进行免疫组化分析,发现CRK高表达与患者预后不良显著相关。进一步机制研究表明,CRK抗体靶向干预可抑制肿瘤微环境中的基质金属蛋白酶活性。

4. **文献名称**: "Functional characterization of CRK isoforms using isoform-specific antibodies in neuronal development"

**作者**: Müller R, Schmidt A.

**摘要**: 研究利用CRK-I和CRK-II亚型特异性抗体,揭示了两种异构体在神经元突触形成中的差异作用。CRK-II抗体阻断实验表明其通过结合DOCK180调控轴突导向,而CRK-I主要参与突触稳定性。

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**注**:以上文献信息为模拟创作,实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词如"CRK antibody" "CRK adaptor protein signaling")。

背景信息

The CRK (CT10 Regulator of Kinase) protein family comprises adaptor proteins crucial for intracellular signal transduction, primarily involved in regulating cell proliferation, differentiation, and migration. Initially identified as the product of the v-CRK oncogene in the avian retrovirus CT10. CRK proteins (CRKI and CRKII isoforms) contain SH2 and SH3 domains that mediate protein-protein interactions by binding phosphorylated tyrosine residues and proline-rich motifs, respectively. These interactions link activated receptor tyrosine kinases (e.g., EGFR, MET) to downstream effectors such as SOS, C3G, and DOCK180. thereby modulating pathways like Ras-MAPK, PI3K/AKT, and JNK. Dysregulation of CRK signaling is implicated in cancer progression, metastasis, and therapeutic resistance, with elevated CRK expression observed in multiple malignancies. CRK antibodies are essential tools for detecting CRK isoforms, studying their phosphorylation-dependent interactions (e.g., Y221 in CRKII), and exploring their roles in disease mechanisms. Commonly used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry, these antibodies aid in elucidating CRK's dual roles as a signaling hub and potential biomarker, while also informing therapeutic strategies targeting CRK-associated pathways.

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