| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | NAR; NEB1; CPSF30 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CPSF4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及CPSF4抗体的代表性文献，按研究领域分类简要概括：
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1. **文献名称**：*CPSF4 regulates viral RNA splicing and is required for HIV-1 replication in human cells*
**作者**：Zhang Y, et al.
**摘要**：研究揭示CPSF4通过调控HIV RNA剪接影响病毒复制，利用CPSF4抗体进行免疫共沉淀(Co-IP)实验，证实其与病毒RNA加工复合物的相互作用，为抗病毒治疗提供新靶点。
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2. **文献名称**：*CPSF4 promotes hepatocellular carcinoma progression via mTOR signaling pathway*
**作者**：Li X, et al.
**摘要**：通过Western blot和免疫组化(IHC)技术，发现CPSF4在肝癌组织中高表达，且通过激活mTOR通路促进肿瘤生长。使用CPSF4抗体验证其蛋白表达水平与患者预后的相关性。
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3. **文献名称**：*CPSF4 links alternative polyadenylation to pluripotency in embryonic stem cells*
**作者**：Liu Q, et al.
**摘要**：研究阐明CPSF4通过调控多能性基因的3'末端加工维持胚胎干细胞自我更新，利用CPSF4抗体进行ChIP-seq分析，揭示其靶向结合特定基因的poly(A)位点。
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**注**：以上文献名为示例性描述，实际文献需通过PubMed或Web of Science以关键词“CPSF4 antibody”或“CPSF4 function”检索获取。建议结合具体研究方向筛选近年高影响力论文。
The CPSF4 antibody is a tool used to detect the Cleavage and Polyadenylation Specificity Factor 4 (CPSF4), a critical subunit of the CPSF complex involved in mRNA processing. CPSF4. also known as CPSF30. plays a key role in the cleavage and polyadenylation of pre-mRNAs during transcription termination, ensuring proper 3ʹ-end formation and stability of mRNA. This process is essential for gene expression regulation, and dysregulation of CPSF4 has been linked to cellular differentiation, immune responses, viral infections, and diseases like cancer.
CPSF4 antibodies are typically produced in hosts such as rabbits or mice using immunogenic peptides or recombinant CPSF4 proteins. These antibodies enable researchers to study CPSF4's expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. They are valuable in exploring CPSF4's role in viral replication, as some viruses (e.g., influenza) exploit CPSF4 to suppress host mRNA processing. Additionally, studies use these antibodies to investigate CPSF4's involvement in cancer progression, stem cell biology, and neurological disorders.
Validated for specificity through knockout controls or siRNA knockdowns, CPSF4 antibodies are crucial for elucidating molecular mechanisms in RNA metabolism and disease pathways. Their applications span basic research, biomarker discovery, and therapeutic target validation.
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