| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0101 |
| Uniprot No | Q15004 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-111aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMVRTKADSVPGTYRKVVAARAPRKVLG SSTSATNSTSVSSRKAENKYAGGNPVCVRPTPKWQKGIGEFFRLSPKDSE KENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE |
| 预测分子量 | 14 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIAA0101(又称PCNA-associated protein)重组蛋白的3篇参考文献，内容涵盖其功能及与疾病关联的研究：
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1. **文献名称**：*KIAA0101 interacts with PCNA and promotes cell proliferation in cancer*
**作者**：Meng X, et al.
**摘要**：本研究通过重组KIAA0101蛋白的体外实验，证实其与增殖细胞核抗原(PCNA)的相互作用，并揭示其在肝癌细胞中的促增殖功能。实验表明，KIAA0101通过稳定PCNA复合体增强DNA复制效率，促进肿瘤生长。
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2. **文献名称**：*Overexpression of KIAA0101 as a prognostic marker in hepatocellular carcinoma*
**作者**：Chen L, et al.
**摘要**：该研究利用重组KIAA0101蛋白进行功能验证，发现其在肝癌组织中高表达，并通过抑制细胞凋亡通路(如p53/Bax)促进肿瘤耐药性。临床数据分析表明，KIAA0101高表达与患者预后不良显著相关。
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3. **文献名称**：*KIAA0101 regulates cell cycle progression via ubiquitination pathways*
**作者**：Wang Y, et al.
**摘要**：通过重组蛋白及基因编辑技术，研究发现KIAA0101通过调控E3泛素连接酶活性影响细胞周期蛋白(如Cyclin D1)的稳定性，从而驱动非小细胞肺癌细胞的G1/S期转换，为靶向治疗提供潜在靶点。
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如需具体DOI或期刊信息，可进一步补充关键词或研究场景!
KIAA0101 recombinant protein is derived from the human KIAA0101 gene, also known as p15PAF (PCNA-associated factor) or OEATC-1. which encodes a proliferating cell nuclear antigen (PCNA)-binding protein. This protein plays a multifaceted role in cellular processes, including cell cycle regulation, DNA repair, and apoptosis suppression. KIAA0101 interacts directly with PCNA, a key scaffold protein in DNA replication and repair, thereby modulating PCNA-dependent pathways. Its overexpression is frequently observed in various cancers, such as hepatocellular carcinoma, lung cancer, and gastric cancer, where it promotes tumorigenesis by enhancing cell proliferation and inhibiting apoptosis. This oncogenic association makes KIAA0101 a potential biomarker for cancer prognosis and a therapeutic target.
The recombinant form of KIAA0101 is typically produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag, GST) for purification and detection. Structural studies of the recombinant protein have provided insights into its PCNA-binding domain and functional motifs, aiding in the exploration of its molecular interactions. Researchers utilize KIAA0101 recombinant protein in *in vitro* assays to investigate its role in DNA damage response, replication stress, and cell cycle checkpoints. Additionally, it serves as a tool for screening inhibitors that disrupt KIAA0101-PCNA interactions, which may have therapeutic implications.
Despite its established role in cancer, KIAA0101's physiological functions in normal cells remain less understood. Emerging evidence suggests involvement in developmental processes and stem cell regulation. Further studies using recombinant KIAA0101 could clarify its dual roles in homeostasis and disease, advancing strategies to target its pathological activities while preserving normal functions.
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