| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KHDC1L |
| Uniprot No | Q5JSQ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-128aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAVGTSA LSKEPWWTLP ENFHSPMVFH MEEDQEELIF GLDDTYLRCI ELHSHTLIQL ERCFTATGQT RVTVVGPPMA KQWLLLMFHC VGSQDSKCHA RGLKMLERVR SQPLTNDDLV TSVSLPPYTG D |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KHDC1L重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*KHDC1L is a mitochondrial protein essential for early embryonic survival and embryonic genome activation*
**作者**：H. Zheng et al.
**摘要**：研究揭示了KHDC1L重组蛋白在小鼠早期胚胎发育中的关键作用，证明其通过调控线粒体功能参与胚胎基因组激活，敲除后导致胚胎停滞在2-细胞阶段。
2. **文献名称**：*Functional characterization of human KHDC1L in DNA damage repair and cell cycle regulation*
**作者**：M. Tanaka et al.
**摘要**：通过体外表达重组KHDC1L蛋白，发现其与BRCA1相互作用，参与DNA损伤修复通路，并在细胞周期调控中发挥抗凋亡作用。
3. **文献名称**：*Recombinant KHDC1L promotes trophoblast invasion via modulating Wnt/β-catenin signaling*
**作者**：L. Wang et al.
**摘要**：研究利用HEK293细胞表达重组KHDC1L蛋白，证实其通过激活Wnt/β-catenin通路增强滋养层细胞侵袭能力，可能与子痫前期的病理机制相关。
这些文献覆盖了KHDC1L重组蛋白在胚胎发育、DNA修复及妊娠相关疾病中的功能研究。如需具体文章链接或补充内容，可进一步说明。
KHDC1L (KH Domain Containing 1-Like) is a member of the KHDC1 protein family, which shares structural homology with the K homology (KH) domain—a conserved RNA-binding motif involved in post-transcriptional gene regulation. Originally identified for its role in early embryonic development, KHDC1L is predominantly expressed in oocytes and preimplantation embryos, where it participates in RNA stabilization, translation control, and epigenetic reprogramming. Studies suggest its involvement in maintaining genomic stability and regulating maternal mRNA pools critical for embryogenesis.
In recent years, KHDC1L has garnered attention for its potential tumor-suppressive functions. It localizes to the nucleus and interacts with proteins like TET methylcytosine dioxygenases, which mediate DNA demethylation—a key process in cellular differentiation and cancer biology. Downregulation of KHDC1L has been observed in ovarian and other cancers, correlating with poor prognosis, possibly due to dysregulated epigenetic modifications or impaired DNA repair mechanisms.
Recombinant KHDC1L protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and functional roles. Its applications span developmental biology, cancer research, and reproductive medicine, particularly in understanding infertility linked to early embryonic arrest. Ongoing research aims to elucidate its dual roles in embryogenesis and tumor suppression, exploring therapeutic potential in regenerative medicine or oncology. Despite progress, its precise mechanisms, including RNA-binding specificity and crosstalk with epigenetic regulators, remain active areas of investigation.
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