| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KISS1 |
| Uniprot No | Q15726 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-138aa |
| 氨基酸序列 | E PLEKVASVGN SRPTGQQLES LGLLAPGEQS LPCTERKPAA TARLSRRGTS LSPPPESSGS PQQPGLSAPH SRQIPAPQGA VLVQREKDLP NYNWNSFGLR FGKREAAPGN HGRSAGRG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《Recombinant KISS1 Protein Expression and Purification in E. coli》**
- 作者：Manfredo, A. et al.
- 摘要：研究报道了利用大肠杆菌系统高效表达KISS1重组蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并验证其体外激活KISS1R受体的生物活性。
2. **《Kisspeptin-54: A Recombinant Form for Therapeutic Potential in Reproductive Disorders》**
- 作者：Dhillo, W.S. et al.
- 摘要：探讨了重组KISS1衍生肽Kisspeptin-54的制备及其在治疗低促性腺激素性性腺功能减退症中的应用，通过临床试验证明其可有效刺激促性腺激素释放。
3. **《Functional Characterization of Recombinant Human Kisspeptin in a Mammalian Cell System》**
- 作者：George, J.T. et al.
- 摘要：研究通过哺乳动物细胞表达系统(CHO细胞)生产重组人KISS1蛋白，验证其结合下丘脑神经元受体并激活下游信号通路的能力，支持其在神经内分泌研究中的应用。
4. **《A Novel Approach for Large-Scale Production of Kisspeptin-10 Using Fusion Protein Technology》**
- 作者：Zhang, L. et al.
- 摘要：提出一种基于融合蛋白策略的大规模Kisspeptin-10重组生产方法，通过酶切分离获得活性肽，显著提高产量并降低成本，适用于工业化制备。
(注：以上文献信息为示例性概括，实际引用需根据具体论文内容调整。)
KISS1 recombinant protein is derived from the KISS1 gene, which encodes a precursor protein cleaved into bioactive peptides called kisspeptins. Discovered initially as metastasis suppressors in cancer, KISS1 gained prominence for its critical role in regulating the reproductive axis. Kisspeptins, particularly kisspeptin-54 (KP-54) and kisspeptin-10 (KP-10), act as potent agonists of the G protein-coupled receptor GPR54 (KISS1R), triggering gonadotropin-releasing hormone (GnRH) secretion. This mechanism is essential for initiating puberty, maintaining reproductive function, and modulating sex steroid feedback.
Recombinant KISS1 proteins are engineered using expression systems like *E. coli* or mammalian cells to produce standardized, high-purity peptides for research and therapeutic applications. They typically include the conserved C-terminal amidated decapeptide region critical for receptor binding. Studies utilize these proteins to explore kisspeptin's roles in fertility disorders, polycystic ovary syndrome (PCOS), hypogonadism, and hormone-dependent cancers. Clinically, KISS1 recombinant proteins are being tested to stimulate ovulation in IVF protocols or treat puberty-related disorders.
Beyond reproduction, emerging research links kisspeptins to metabolic regulation, bone homeostasis, and brain function, highlighting their systemic influence. However, challenges remain in optimizing stability and delivery methods for therapeutic use. Recombinant technology enables scalable production and structural modifications to enhance bioavailability or receptor specificity, driving both mechanistic studies and drug development. As a key neuroendocrine regulator, KISS1 recombinant proteins continue to bridge translational gaps in endocrinology and oncology.
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