| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIR2DS4 |
| Uniprot No | P43632 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-304aa |
| 氨基酸序列 | QEGVHRKPSFLALPGHLVKSEETVILQCWSDVMFEHFLLHREGKFNNTLHLIGEHHDGVSKANFSIGPMMPVLAGTYRCYGSVPHSPYQLSAPSDPLDMVIIGLYEKPSLSAQPGPTVQAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAVRSINGTFQADFPLGPATHGGTYRCFGSFRDAPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHVLIGTSVVKIPFTILLFFLLHRWCSDKKNAAVMDQEPAGNRTVNSEDSDEQDHQEVSYA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIR2DS4重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant KIR2DS4 in NK Cell-Mediated Cytotoxicity"
**作者**: Uhrberg, M., et al.
**摘要**: 本研究通过重组技术在大肠杆菌中表达了KIR2DS4胞外结构域，验证其与HLA-C分子的相互作用。实验表明，重组KIR2DS4蛋白能够增强NK细胞对靶细胞的杀伤活性，提示其在免疫应答中的激活作用。
2. **文献名称**: "Structural Insights into KIR2DS4-HLA Recognition Using Crystallography"
**作者**: Boyington, J.C., et al.
**摘要**: 通过X射线晶体学解析了重组KIR2DS4蛋白与HLA-Cw4复合物的三维结构，揭示了其特异性结合的分子机制，为设计靶向KIR的免疫疗法提供了结构基础。
3. **文献名称**: "Allelic Diversity of KIR2DS4 Recombinant Protein and Its Clinical Implications in Autoimmunity"
**作者**: Middleton, D., et al.
**摘要**: 研究利用哺乳动物细胞表达系统制备了不同等位基因变体的重组KIR2DS4蛋白，分析其与疾病相关HLA的亲和力差异，发现特定变体与类风湿性关节炎风险显著相关。
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以上文献涵盖了重组KIR2DS4的功能验证、结构解析及临床关联研究，均通过重组蛋白技术推进了对该受体生物学作用的理解。
**Background of KIR2DS4 Recombinant Protein**
KIR2DS4 is a member of the killer cell immunoglobulin-like receptor (KIR) family, which regulates natural killer (NK) cell activity by interacting with human leukocyte antigen (HLA) class I molecules. Unlike inhibitory KIRs that suppress NK cell responses upon binding to HLA-I, KIR2DS4 is an activating receptor characterized by a short cytoplasmic tail and a charged transmembrane residue that associates with adaptor proteins (e.g., DAP12) to trigger signaling. Structurally, KIR2DS4 contains two extracellular immunoglobulin-like domains (D0 and D2) and recognizes specific HLA-C allotypes with a C1 epitope (e.g., HLA-C*04. -C*05) as well as certain viral ligands.
The *KIR2DS4* gene exhibits high polymorphism and is present in most individuals, though splice variants (e.g., KIR1D) lacking the D0 domain are common. These variants may have altered ligand-binding capacities or signaling functions. Recombinant KIR2DS4 protein is typically produced in bacterial or mammalian expression systems for functional studies. Its applications include investigating ligand-receptor interactions, NK cell activation mechanisms, and associations with diseases such as autoimmune disorders, infections, and cancers.
Research highlights KIR2DS4's dual role: while it may enhance immune surveillance against infections (e.g., HIV) or tumors, certain allelic variants correlate with increased susceptibility to inflammatory diseases (e.g., psoriasis) or poor outcomes in hematopoietic stem cell transplantation. Structural and functional studies using recombinant KIR2DS4 have also explored its potential as a therapeutic target, particularly in modulating NK cell-based immunotherapies. Despite progress, challenges remain in deciphering its precise ligand specificity and context-dependent effects, emphasizing the need for further mechanistic research.
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