| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0513 |
| Uniprot No | O60268-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-401aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMETPEVPVGSLIDFGPEAPTSSPLEAP PPVLQDGDGSLGDGASESETTESADSENDMGESPSHPSWDQDRRSSSNES FSSNQSTESTQDEETLALRDFMRGYVEKIFSGGEDLDQEEKAKFGEYCSS ENGKGREWFARYVSAQRCNSKCVSEATFYRLVQSFAVVLFECHQMDDFGP AKNLMTMCFTYYHIGKPQLLPPESREKPAGSIDSYLKSANSWLAEKKDIA ERLLKNTSARTENVKGFFGGLETKLKGPLARRNEEDENKPQEKRPRAVTA YSPEDEKKGEKIYLYTHLKQQPIWHTLRFWNAAFFDAVHCERTKRSPTTR EKWCHMTQEERDDSLRFNENITFGQLGTFTHNMLAFGLNKKLCNDFLKKQ AVIGNLDEEQYKLLSDHIEQMATE |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIAA0513(HIPK1)重组蛋白的3篇参考文献概要：
1. **文献名称**: *"Molecular cloning and characterization of a novel protein kinase, KIAA0513"*
**作者**: Kawai J., et al.
**摘要**: 该研究首次从人脑cDNA文库中克隆了KIAA0513基因，并通过序列分析预测其编码一种丝氨酸/苏氨酸蛋白激酶。重组蛋白在体外实验中显示激酶活性，提示其在细胞信号转导中的潜在功能。
2. **文献名称**: *"HIPK1 modulates DNA damage response by phosphorylating p53 at Ser46"*
**作者**: Kim Y., et al.
**摘要**: 研究通过重组表达人源HIPK1(KIAA0513)蛋白，证实其通过磷酸化p53蛋白的Ser46位点参与DNA损伤应答调控。实验表明，HIPK1重组蛋白的激酶活性对细胞凋亡有促进作用。
3. **文献名称**: *"Structural and functional analysis of the HIPK1 kinase domain"*
**作者**: Sánchez-Pulido L., et al.
**摘要**: 该文献解析了KIAA0513/HIPK1的激酶结构域，利用重组蛋白进行体外酶活性和底物筛选实验，揭示了其催化机制及与底物结合的关键氨基酸残基，为靶向药物设计提供基础。
4. **文献名称**: *"HIPK1 overexpression correlates with cancer progression and poor prognosis"*
**作者**: Calzado M.A., et al.
**摘要**: 研究通过重组HIPK1蛋白及细胞模型，证明其在肿瘤细胞增殖和迁移中的作用，并发现HIPK1高表达与患者预后不良相关，提示其作为癌症治疗靶点的潜力。
(注：以上文献标题及作者为模拟示例，实际文献需通过数据库核实。)
KIAA0513. also known as HIPK4 (Homeodomain-interacting protein kinase 4), is a serine/threonine kinase belonging to the HIPK family. This protein is encoded by the KIAA0513 gene in humans and is evolutionarily conserved across species. HIPK4 shares structural homology with other HIPK members, including a catalytic kinase domain and regions implicated in protein-protein interactions. While its biological functions are less characterized compared to HIPK1-3. emerging studies suggest roles in cellular processes such as transcriptional regulation, apoptosis, and stress signaling. HIPK4 is expressed in various tissues, with higher levels observed in the testis, brain, and heart, hinting at tissue-specific regulatory functions.
Recombinant KIAA0513/HIPK4 protein is engineered for in vitro studies to elucidate its molecular mechanisms. Typically produced in bacterial or mammalian expression systems, the recombinant protein retains kinase activity and is often tagged (e.g., His, GST, or FLAG tags) to facilitate purification and detection. Its production enables biochemical assays to identify substrates, map phosphorylation sites, and screen kinase inhibitors. Structural studies using recombinant HIPK4 have provided insights into its ATP-binding pocket and potential regulatory domains, aiding drug discovery efforts.
Recent research links HIPK4 to spermatogenesis and neuronal development. In mice, HIPK4 knockout models display male infertility due to defective sperm maturation, suggesting its critical role in germ cell differentiation. Additionally, HIPK4 may interact with signaling pathways like Wnt or TGF-β, though precise targets remain under investigation. Dysregulation of HIPK4 has been tentatively associated with cancers and neurodevelopmental disorders, highlighting its potential as a therapeutic target. Recombinant HIPK4 thus serves as a vital tool for unraveling its physiological and pathological significance.
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