| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/400 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EG1; magicin; 1500003D12Rik |
| WB Predicted band size | 19 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MED28 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于MED28抗体的3篇参考文献示例，包含文献名称、作者及摘要概述：
1. **"MED28 Expression in Breast Cancer: Correlation with Clinicopathological Parameters"**
*作者：Smith A, et al.*
摘要：本研究通过免疫组化分析乳腺癌组织中MED28蛋白的表达水平，发现其高表达与肿瘤侵袭性和不良预后相关，提示MED28可能作为乳腺癌的生物标志物。
2. **"MED28 Regulates TGF-β Signaling via Interaction with Smad Proteins"**
*作者：Zhang L, et al.*
摘要：文章利用MED28抗体进行Western blot和免疫共沉淀实验，揭示MED28通过结合Smad2/3蛋白调控TGF-β信号通路，影响细胞增殖与迁移。
3. **"Structural Role of MED28 in the Mediator Complex Assembly"**
*作者：Johnson R, et al.*
摘要：通过免疫荧光和抗体阻断实验，研究发现MED28在中介体复合体的亚细胞定位和稳定性中起关键作用，其缺失导致转录调控功能受损。
4. **"Development and Validation of a Novel Anti-MED28 Monoclonal Antibody for Diagnostic Applications"**
*作者：Chen H, et al.*
摘要：研究团队开发了一种高特异性抗MED28单克隆抗体，验证其在ELISA和免疫组化中的应用效能，为MED28相关疾病的诊断提供新工具。
*注：以上文献为示例性质，实际引用时请核实真实文献信息。*
MED28. a subunit of the Mediator complex, plays a critical role in transcriptional regulation by bridging transcription factors and RNA Polymerase II. This evolutionarily conserved multiprotein complex is essential for modulating gene expression across eukaryotes. MED28 is part of the Mediator’s regulatory "kinase module," though its precise functional interactions remain under investigation. Studies suggest its involvement in cellular processes like proliferation, differentiation, and stress responses, with dysregulation linked to cancers and cardiovascular diseases.
MED28 antibodies are vital tools for exploring its molecular mechanisms and expression patterns. They enable detection and localization of MED28 in various experimental approaches, including Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Researchers utilize these antibodies to study MED28’s role in disease contexts, particularly its potential as a biomarker or therapeutic target. Commercial MED28 antibodies are typically raised against specific epitopes (e.g., human MED28 N-terminal regions) and validated for cross-reactivity in model organisms. However, batch variability and validation rigor remain considerations for experimental reproducibility. Ongoing research aims to clarify MED28’s tissue-specific functions and its interplay with other Mediator subunits in transcriptional programs.
×
