Rabbit Polyclonal POFUT1 Antibody

The POFUT1 antibody is a tool used to detect and study Protein O-fucosyltransferase 1 (POFUT1), an enzyme critical for post-translational modification of proteins. POFUT1 catalyzes the addition of O-linked fucose to specific serine/threonine residues in epidermal growth factor-like (EGF) repeats of proteins, notably within the Notch signaling pathway. This modification is essential for proper folding, trafficking, and function of Notch receptors, which play pivotal roles in cell differentiation, development, and homeostasis. Dysregulation of POFUT1 has been implicated in various diseases, including cancer, cardiovascular disorders, and Dowling-Degos disease, a genetic skin condition linked to POFUT1 mutations. Researchers use POFUT1 antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and its relationship to disease mechanisms. These antibodies are often validated in knockout cell lines or tissues to ensure specificity. Given the therapeutic potential of targeting Notch signaling, POFUT1 antibodies also aid in exploring novel treatments. Their applications extend to developmental biology, oncology, and genetic research, providing insights into how O-fucosylation influences cellular communication and disease progression.